Transcriptomics,Genomics

Dataset Information

25

Transcriptomic analysis of APC knockdown in proliferating primary myoblasts


ABSTRACT: APC is a key regulator of canonical Wnt signalling since it participates to beta-catenin targeting to proteasomal degradation when the pathway is inactive. Moreover, independently of Wnt signaling, APC regulates several cellular functions such as mycrotubule dynamics, chromosome segregation, cell adhesion. Although APC has been widely studied for its implication in initation and progression of several cancers, its role in satellite cells (skeletal muscle stem cells) has never been investigated. Here we used microarrays and to clarify APC functions and we identified several pathways and cellular processes to be affected following APC silencing. Overall design: We cultured satellite cells-derived primary myoblasts in proliferating conditions and we transfected them either by a siRNA targeting APC or by a control siRNA. 48 hours after transfection, cells were processed for RNA extraction.

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: mogene10st_Mm_ENTREZG_17.1.0]

SUBMITTER: Fabien Le Grand  

PROVIDER: GSE57898 | GEO | 2015-01-01

SECONDARY ACCESSION(S): PRJNA248381

REPOSITORIES: GEO

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Publications

APC is required for muscle stem cell proliferation and skeletal muscle tissue repair.

Parisi Alice A   Lacour Floriane F   Giordani Lorenzo L   Colnot Sabine S   Maire Pascal P   Le Grand Fabien F  

The Journal of cell biology 20150824 5


The tumor suppressor adenomatous polyposis coli (APC) is a crucial regulator of many stem cell types. In constantly cycling stem cells of fast turnover tissues, APC loss results in the constitutive activation of a Wnt target gene program that massively increases proliferation and leads to malignant transformation. However, APC function in skeletal muscle, a tissue with a low turnover rate, has never been investigated. Here we show that conditional genetic disruption of APC in adult muscle stem c  ...[more]

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