Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome Sequencing (RNA-seq) of Normal Human Osteoblasts

ABSTRACT: Three normal human osteoblast samples, acquired from PromoCell, were used as controls to compare to RNA-seq data from prepublished osteosarcoma samples (submitted to the European Bioinformatics Institute; EGAS00001000263) for the purpose of evaluating expression levels of genes identified as common insertions sites in a Sleeping Beauty screen of osteosarcomas in mice.

ORGANISM(S): Homo sapiens

PROVIDER: GSE57925 | GEO | 2015/04/06

SECONDARY ACCESSION(S): PRJNA248432

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome Sequencing (RNA-seq) of Normal Human Osteoblasts

Project description:Three normal human osteoblast samples, acquired from PromoCell, were used as controls to compare to RNA-seq data from prepublished osteosarcoma samples (submitted to the European Bioinformatics Institute; EGAS00001000263) for the purpose of evaluating expression levels of genes identified as common insertions sites in a Sleeping Beauty screen of osteosarcomas in mice. Three normal human osteoblast samples (pellet form in RNAlater) were acquired from PromoCell (Heidelberg, Germany), and RNA was isolated from them immediately upon receipt.

2015-04-06 | E-GEOD-57925 | biostudies-arrayexpress

Comparative Genome-Wide Profiling of Transposon and Retrovirus Integration Sites in Primary Human CD4+ T Cells

Project description:We mapped Sleeping Beauty (SB) and piggyBac (PB) insertions in primary human CD4+ T cells and compared their insertion profiles with those of the MLV retrovirus and the HIV lentivirus with respect to proximity to genes, TSSs, CpG islands, DNase I hypersensitive sites, repetitive elements, chromatin marks and transcriptional status of genes. Examination of two transposon systems in one cell type.

2015-07-01 | E-GEOD-58744 | biostudies-arrayexpress

Comparative Genome-Wide Profiling of Transposon and Retrovirus Integration Sites in Primary Human CD4+ T Cells

2015-07-01 | GSE58744 | GEO

Cell of origin strongly influences genetic selection in a mouse model of T-ALL.

Project description:Thymic lymphomas were generated by inducing Sleeping Beauty transposon mutagenesis at different stages of T-cell development. This dataset includes exon array results from 14 tumor samples from two different Sleeping Beauty models of T-ALL (7 Vav-SB and 7 CD4-SB samples).

2011-08-20 | E-GEOD-29849 | biostudies-arrayexpress

Homo sapiens

Project description:Sandwich Sleeping Beauty integrome

| PRJNA261004 | ENA

Therapeutic immune cell engineering with an mRNA : AAV-Sleeping Beauty composite system [Splinkerette]

Project description:We evaluated the insertion site profile of the MAJESTIC (mRNA : AAV-Sleeping Beauty) CAR generation method.

2023-05-18 | GSE220202 | GEO

Cell of origin strongly influences genetic selection in a mouse model of T-ALL.

Project description:Thymic lymphomas were generated by inducing Sleeping Beauty transposon mutagenesis at different stages of T-cell development.

2011-08-21 | GSE29849 | GEO

A Sleeping Beauty Forward Genetic Screen Identifies Genes Promoting Osteosarcoma Development and Metastasis

Project description:A Sleeping Beauty (SB) transposon forward genetic screen was performed to identify the genes that promote osteosarcoma (OS) development and metastasis. Mutagenesis induced OS in wild type mice and accelerated it on a Trp53 deficient background. Analysis of tumors demonstrated that Trp53 deficiency is correlated with genomic instability, which was virtually absent in tumors induced by SB mutagenesis alone. Metastases developed in a subset of animals and in nearly all cases were clonal related to primary tumors. Over 200 candidate genes were identified, many of which are altered in human cancers including OS. Signaling pathways enriched for candidate genes were also identified and a subset of these pathways and genes were functionally validated and represent new targets for OS treatment. Bisulphite converted DNA from the 21 diagnosis osteosarcoma patients and 3 hOB cell line replicates were hybridised to the Illumina Infinium 450K Human Methylation Beadchip.

2015-04-07 | E-GEOD-58770 | biostudies-arrayexpress

Gene expression profiling in Osteosarcomas

Project description:To explore whether KSHV infection is a risk factor for osteosarcoma development in ethnic Uyghur population. Sarcoma specimens including tumor, adjacent normal tissues and paired blood samples were obtained from several osteosarcoma patients of Xinjiang China. Then, osteosarcoma clinical samples were subjected to analysis for the presence of the KSHV genome and proteins. Gene expression profiles of these osteosarcomas were analyzed to reveal the pathogenesis of KSHV-positive and -negative osteosarcomas.

2019-09-01 | GSE126209 | GEO

Homo sapiens

Project description:A Sleeping Beauty Forward Genetic Screen Identifies Genes Promoting Osteosarcoma Development and Metastasis

| PRJNA253411 | ENA