Project description:Interventions: Group 1:Total fluid input = physiological requirement + preoperative cumulative loss + continuing loss + third space loss + compensatory volume expansion;Group 2:Goal-directed fluid therapy based on stroke volume variation;Group 3:Goal-directed fluid therapy based on stroke volume variation and plasma colloid osmotic pressure
Primary outcome(s): Indicators of changes in lung function;Postoperative pulmonary complications occurring within 7 days after surgery;Lung injury score
Study Design: Parallel
Project description:Elevated temperatures due to global warming seriously threaten crops production. Understanding molecular mechanisms of cellular responses to heat stress will help to improve crop tolerance to high temperature and yield. In this work, we show that deacetylation of non-histone proteins mediated by the rice cytoplasmic histone deacetylase HDA714 is required for plant tolerance to heat stress. HDA714 expression and protein accumulation are induced by heat stress. HDA714 loss-of-function affects specifically plant response to heat (42°C) while its over-expression enhances plant tolerance to the stress. Interestingly, heat stress led to decreases of overall protein lysine acetylation in rice plants, which depends on HDA714 function. HDA714-mediated deacetylation of metabolic enzymes stimulates glycolysis under heat stress. In addition, HDA714 protein is found within heat-induced stress granules (SGs), many identified rice SG proteins show lysine acetylation at normal temperature (25 °C), which is augmented in hda714 mutants. Finally, HDA714 interacts with and deacetylates several SG proteins and HDA714 loss-of-function impairs SG formation. Collectively, these results indicate that HDA714-mediated cellular protein lysine deacetylation responds to heat stress, affects metabolic activities, regulates SGs formation, and confers heat tolerance in rice plants.
Project description:In this study, we identified a new rice loss-of-function mutant, small organ size1 (smos1), that decreases the final size of various organs due to decreased cell size and abnormal microtubule orientation. SMOS1 encodes an unusual APETALA2 (AP2)-type transcription factor with an imperfect AP2 domain, and its product belongs to the basal AINTEGUMENTA (ANT) lineage, including WRINKLED1 (WRI1) and ADAP. SMOS1 expression was induced by exogenous auxin treatment, and the auxin response element (AuxRE) of the SMOS1 promoter acts as a cis-motif through interaction with auxin response factor (ARF). Furthermore, a functional fluorophore-tagged SMOS1 was localized to the nucleus, supporting the role of SMOS1 as a transcriptional regulator for organ size control. Microarray analysis showed that the smos1 mutation represses expression of several genes involved in microtubule-based movement and DNA replication. Expression profiling with the rice 44K-Agilent microarray was conducted using RNAs extracted from the leaf sheaths of WT and smos1 mutant. Each dataset was obtained from three biological replicates.
Project description:We performed genome-wide profiling experiments on tissues from Drosophila melanogaster adult females with a mutation in the p24 gene logjam (loj) to test the hypothesis that loss of loj function causes a transcriptional response characteristic of ER stress activation. Keywords: logjam mutant compared to control
Project description:Heading date1(Hd1) is a critical regulator controlling rice flowering time, which promotes flowering under short-day (SD) conditions and represses flowering under long-day (LD) conditions. In our previous study (Luan et al., 2009), we identified a rice mutant, hd1-3, in which the Hd1 gene was deficient due to several insertions/deletions in the coding region. To search for downstream genes regulated by Hd1, we performed microarray analysis of hd1-3 mutant and the wild-type Zhonghua11 under both SD and LD conditions. According to the microarray results, SDG712 gene was significantly downregulated in the hd1-3 mutant, indicating that SDG712 gene may acts downstream of Hd1, and may functions in rice flowering time regulation.
Project description:Transcriptomic analysis was performed on the main inflorescence stems of wild-type and lignin-modified lines growing under the same conditions. Study transcriptomic changes in arabidopsis stem manipulated in F5H. Loss of function fah-1 mutant and F5H overexpression (F5H-OE) We used microarrays to detail the global determine changes of gene expression in loss of function fah-1 mutant and F5H overexpression (F5H-OE) up and down-regulated genes were identified.
Project description:Copy number variations (CNVs) can create new genes, change gene dosage, reshape gene structures, and modify elements regulating gene expression. As with all types of genetic variation, CNVs may influence phenotypic variation and gene expression. CNVs are thus considered major sources of genetic variation. Little is known, however, about their contribution to genetic variation in rice. To detect CNVs, we used a set of NimbleGen whole-genome comparative genomic hybridization arrays containing 715,851 oligonucleotide probes with a median probe spacing of 500 bp. We compiled a high-resolution map of CNVs in the rice genome, showing 641 CNVs between the genomes of the rice cultivars ‘Nipponbare’ (from O. sativa ssp. japonica) and ‘Guang-lu-ai 4’ (from O. sativa ssp. indica). These CNVs contain some known genes. They are linked to variation among rice varieties, and are likely to contribute to subspecific characteristics.