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Separation of mouse ES cell-derived mesodermal cells based on the expression of Flk1, PDGFRa and CAR.

ABSTRACT: We found that mouse ES cell-derived Flk1+ cells could be subdivided into three population by the expression of PDGFRa and CAR (Flk1+PDGFRa-CAR-, Flk1+PDGFRa-CAR+, and Flk1+PDGFRa+CAR+). Therefore, global gene expression analysis was perfomed by microarray to characterize these mesodermal subsets.

ORGANISM(S): Mus musculus

PROVIDER: GSE58170 | GEO | 2015/05/22

SECONDARY ACCESSION(S): PRJNA251430

REPOSITORIES: GEO

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Separation of mouse ES cell-derived mesodermal cells based on the expression of Flk1, PDGFRa and CAR.

Project description:We found that mouse ES cell-derived Flk1+ cells could be subdivided into three population by the expression of PDGFRa and CAR (Flk1+PDGFRa-CAR-, Flk1+PDGFRa-CAR+, and Flk1+PDGFRa+CAR+). Therefore, global gene expression analysis was perfomed by microarray to characterize these mesodermal subsets. RNA isolated from five separate experiments was pooled and used for comparison

2015-05-22 | E-GEOD-58170 | biostudies-arrayexpress

Expression data from lateral and paraxial mesodermal cells differentiated from mouse Embryonic Stem Cells

Project description:Mouse Embryonic Stem Cells (mESCs) were differentiated with EB formation and characterized with Flk1 and PDGFαR specific antibodies. miRNA profile of lateral mesodermal cells, paraxial mesodermal cells, DN (Double negative) and DP (Double positive) populations were firstly determined and effects of differentially expressed miRNAs transfected transiently on EB formation, and myogenic and hematopoietic differentiation potential were assessed.

2021-07-07 | GSE156238 | GEO

Mus musculus

Project description:Separation of mouse ES cell-derived mesodermal cells based on the expression of Flk1, PDGFRa and CAR.

| PRJNA251430 | ENA

Changes in Flk1-positive endothelial cells in hypoxia [GFP]

Project description:To investigate the role of Flk1 in pulmonary arterial hypertension, we observed the behavior of Flk1 in a disease model using Flk1-GFP mice. Gene expression profiling analysis was performed using data obtained from RNA-seq of Flk1-positive cells on days 1 and 8 of hypoxia exposure, when Flk1 changes were greatest.

2023-08-25 | GSE216681 | GEO

Role of Flk1 in pulmonary arterial hypertension [Flox]

Project description:To investigate the role of Flk1 in pulmonary arterial hypertension, we generated mice lacking Flk1 specifically in vascular endothelial cells. We exposed these mice to hypoxia and collected Flk1-KO endothelial cells by FACS for RNA-seq.

2023-08-25 | GSE216682 | GEO

Expression data from differentiating Flk1- and Flk1+ ES cells expressing Snail during Wnt inhibition

Project description:ES cells differentiated in the presence of the Wnt inhibitor DKK1 fail to express the transcription factor Snail and undergo EMT or mesoderm differentiation. We generated an ES cell line, A2.snail, that induced Snail expression upon addition of doxycycline addition. Microarrays were used to gain a global picture of Flk1- and Flk1+ cells generated one day after Snail was expressed during Wnt inhibition. A2.snail ES cells, which express Snail upon addition of doxycycline, were differentiated as embryoid bodies in differentiation media and DKK1. Snail-induced cultures uniquely develop a select population of Flk1+ cells. Total RNA was harvested from sorted control (no doxycycline) Flk1- cells and sorted Snail-induced (doxycycline at day 2) Flk1- and Flk1+ cells at day 3 of differentiation.

2013-10-23 | E-GEOD-26524 | biostudies-arrayexpress

Expression data from differentiating Flk1- and Flk1+ ES cells expressing Snail during Wnt inhibition

2013-10-23 | GSE26524 | GEO

Expression data of control (F/F) and Snai1 knockout (KO) Flk1+/- cells

Project description:Snai1 is a master factor of epithelial to mesenchymal transitioin (EMT), however, its role in embryonic stem cell (ESC) differentiation and lineage commitment remains undefined. We used microarrays to compare the global programme of gene expression between control and Snai1 knockout Flk1+ and Flk1- cells sorted from 4 day EBs. Control and Snai1 knockout ESCs were cultured as embryoid bodies in spotaneous differentiation media, 4 days EBs were dissociated and sorted by anti-Flk1 antibody to separated Flk1+ and Flk1- cells, total RNA were collected for Affymetrix microarrays

2013-12-10 | E-GEOD-53108 | biostudies-arrayexpress

Expression data of control (F/F) and Snai1 knockout (KO) Flk1+/- cells

2013-12-10 | GSE53108 | GEO

Whole transcriptome analysis of reaggregated embryoid bodies treated with IWR-1

Project description:We identified distict mesodermal sub-populations based on Endoglin (Eng) and Flk1 expression in Brachyury (Bry) positive cells. By using whole-transcriptome analysis, we further characterized these populations and how they changed when Wnt pathway is inhibited Reaggregates mRNA profiles of unsorted, Flk1+ Eng+, and Flk1- Eng+ samples were generated by deep sequencing, in triplicate , using Ilumina.

2016-08-20 | E-GEOD-85855 | biostudies-arrayexpress

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