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PROTEOFORMER: deep proteome coverage through ribosome profiling and MS integration

ABSTRACT: An increasing amount of studies integrate mRNA sequencing data into MS-based proteomics to complement the translation product search space. We present the generation of a protein synthesis-based database from deep sequencing of ribosome-protected mRNA fragments. This approach increases the overall protein identification rates with 3% and 11% (improved and new identifications) for human and mouse respectively and enables proteome-wide detection of 5’-extended proteoforms, uORF translation and near-cognate translation start sites.

ORGANISM(S): Homo sapiens

PROVIDER: GSE58207 | GEO | 2014/12/01

SECONDARY ACCESSION(S): PRJNA251587

REPOSITORIES: GEO

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