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Gene expression profiling of PBMC from normal individuals, chronic hepatitis B carriers and hepatocellular carcinoma patients

ABSTRACT: Aberrant gene expression analysis between peripheral blood mononuclear cell (PBMC) samples from healthy individuals and patients with chronic hepatitis B carriers and HCC were identified using Affymetrix gene arrays.

ORGANISM(S): Homo sapiens

PROVIDER: GSE58208 | GEO | 2014/06/04

SECONDARY ACCESSION(S): PRJNA251591

REPOSITORIES: GEO

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Gene expression profiling of PBMC from normal individuals, chronic hepatitis B carriers and hepatocellular carcinoma patients

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Microarray gene expression from hepatitis B virus infection to hepatocellular carcinoma

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Expression profiling of PBMC from patients with hepatocellular carcinoma

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Comparative Transcriptome Analysis of Peripheral Blood Mononuclear Cells Across Clinical Cohorts of Hepatitis B Virus Induced Hepatocellular Carcinoma and Chronic Asymptomatic Hepatitis B Infection in Nigeria

Project description:Hepatitis B Virus (HBV) infection affects about 20 million Nigerians and its most fatal complications is the hepatocellular carcinoma (HCC). Chronic hepatitis B (CHB) infection progresses to HCC which lacks sensitive and specific biomarkers at early stage for detection and subsequent treatment. Efforts in this study is therefore to assess the differential gene expression across clinical cohorts of HBV infection to identify the potential biomarkers of early-stage HCC. Total RNA was extracted and the peripheral blood mononuclear cells (PBMC) in 12 samples were sequenced through the bulk RNA sequencing modality. This was followed by bioinformatic analysis for differential gene expression studies using GALAXY, Qiagen RNA-Sequencing Analysis and DAVID pipelines. Transcriptomic analysis of the PBMC identified a total of 66,726 genes and transcripts. Among patients with HBV-Induced HCC vs CHB, a total of 3,204 genes were differentially expressed with 76 and 272 genes significantly upregulated and downregulated respectively. Across the clinical cohorts of HBV-induced HCC vs healthy controls, 4561 were dysregulated with 370 and 375 genes significantly upregulated and downregulated respectively. Across the significantly upregulated genes of the cohort HBV-induced HCC vs healthy controls, 86 Gene ontology (GO) terms were identified, 19 clustered, 103 terms did not cluster, while across the downregulated cohort, 255 GO terms were identified, 63 clustered, 335 did not. The mitogen-activated protein kinase (MAPK) signalling pathway, cell division cycle and microRNA pathways in the HCC cohort were dysregulated

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Prophylactic or Preemptive Entecavir in Patients With Colorectal Cancer Who Are Inactive Hepatitis B Carriers

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Project description:Gene expression profiling of hepatocellular carcinoma (HCC) and background liver has been studied extensively; however, the relationship between the gene expression profiles of different lesions has not been assessed. We examined the expression profiles of 34 HCC specimens (17 hepatitis B virus [HBV]-related and 17 hepatitis C virus [HCV]-related) and 71 non-tumor liver specimens (36 chronic hepatitis B [CH-B] and 35 chronic hepatitis C [CH-C]) using an in-house cDNA microarray consisting of liver-predominant genes. Graphical Gaussian modeling (GGM) was applied to elucidate the interactions of gene clusters among the HCC and non-tumor lesions. Gene expression profiling of HCC and non-tumor lesions revealed the predisposing changes of gene expression in HCC. This approach has potential for the early diagnosis and possible prevention of HCC. We examined the expression profiles of 34 HCC specimens (17 hepatitis B virus [HBV]-related and 17 hepatitis C virus [HCV]-related) and 71 non-tumor liver specimens (36 chronic hepatitis B [CH-B] and 35 chronic hepatitis C [CH-C]) using an in-house cDNA microarray consisting of liver-predominant genes. Graphical Gaussian modeling (GGM) was applied to elucidate the interactions of gene clusters among the HCC and non-tumor lesions.

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Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma

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