Genomics

Dataset Information

162

The LSD1/KDM1A neuro-specific isoform regulates neuronal differentiation through H3K9 demethylation [ChIP-Seq]


ABSTRACT: During brain development, histone-modifying enzymes play an important role by orchestrating transcriptional programs that regulate neuronal maturation. Lysine-Specific Demethylase 1 (LSD1, also named as KDM1A) functions as a transcriptional repressor by removing methyl groups at lysine 4 of histone H3 (H3K4). In neurons, alternative splicing can include an additional exon (exon E8a) within LSD1 transcripts, generating a LSD1+8a neuro-specific isoform. We here report that LSD1+8a isoform does not have the intrinsic ability to demethylate H3K4. LSD1+8a functions as a co-activator on its target genes by removing H3K9 repressive histone marks. We identify the supervillin protein (SVIL) as a LSD1+8a interacting partner and demonstrate that SVIL protein regulates neuronal maturation by controlling LSD1+8a mediated H3K9 demethylation. Thus, our results show that alternative splicing provides a genius mechanism by which LSD1 isoforms can acquire a dual specificity (H3K9 vs H3K4) and therefore differentially control specific gene expression patterns during brain development. Overall design: Examination of 2 different LSD1 isoforms LSD1 and LSD1+8a, LSD1+8a's cofactor SVIL, H3K9me2 in SH-SY5Y cells infected with control or LSD1+8a shRNA during neuronal maturation induced with BNDF at Day0(B0) and Day3(B3).ChIP-seq datas were generated by deep sequencer Hiseq2500.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

SUBMITTER: Shi Yang  

PROVIDER: GSE58258 | GEO | 2015-06-05

SECONDARY ACCESSION(S): PRJNA251753

REPOSITORIES: GEO

Similar Datasets

2015-06-05 | E-GEOD-63097 | ArrayExpress
2013-06-25 | E-GEOD-40440 | ArrayExpress
2014-12-12 | E-GEOD-52201 | ArrayExpress
| GSE36346 | GEO
2012-03-28 | E-GEOD-36346 | ArrayExpress
2015-04-29 | E-GEOD-62770 | ArrayExpress
| GSE77448 | GEO
| GSE68139 | GEO
| GSE75054 | GEO
| PRJNA266820 | ENA