Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Impact of gamma chain cytokines on the differentiation of recently antigen-activated CD8 T cells

ABSTRACT: Analysis of how different gamma chain family cytokines influence CD8 T cell differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE58262 | GEO | 2014/06/06

SECONDARY ACCESSION(S): PRJNA251757

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Impact of gamma chain cytokines on the differentiation of recently antigen-activated CD8 T cells

Project description:Analysis of how different gamma chain family cytokines influence CD8 T cell differentiation. Naïve CD8 T cells were isolated from the spleens OT-I Thy1.1 TCR Tg mice. Whole splenocytes from wild-type C57BL/6 mice were used as stimulator cells. Purified naïve wild-type OT-I (1×106/well) were stimulated with OVA peptide (SIINFEKL) pulsed (5 µg/ml) and irradiated (2,000 rads) syngeneic splenocytes (6×106/well) in 24-well plates. Forty-eight hours later, activated OT-I T cells were harvested and viable cells were enriched over a Ficoll-paque gradient and washed with cRPMI prior to being reseeded in cRPMI (5×105 cells/ml) and treated with the media supplemented with various γc cytokines (IL-2, IL-4, IL-7, IL-15 and IL-21). Experimental samples were treated with 100 ng/ml of their respective gamma chain cytokine and incubated at 37ºC for 24 hours. RNA was isolated using either the RNeasy Mini kit, or a combination of TRIzol reagent and the Direct-zol RNA miniprep kit, all following manufacturer protocols. Two biological replicates for mRNA analysis were prepped using the RNeasy kit. The third replicate was prepped using the Trizol/Direct-zol approach. The quality and quantity of RNA samples was further analyzed on the Bioanalyzer. Labeled target cDNA was prepared from total RNA samples using the Ambion MessageAmp Premier protocol (3’IVT assay). Each sample target was hybridized to a Mouse 430 2.0 GeneChip array. Image processing and expression analysis were performed using Affymetrix GeneChip Command Console (AGCC) v. 3.1.1 and Affymetrix Expression Console v.1.1 software, respectively. Data from all biological replicates and conditions was imported into the Affymetrix Expression Console and normalized (RMA). RNA processing and microarray hybridization were performed by the Oregon Health & Science University Gene Microarray Shared Resource core facility in Portland, Oregon.

2014-06-06 | E-GEOD-58262 | biostudies-arrayexpress

Gamma chain

Project description:Gamma chain microbiota

| PRJEB6359 | ENA

ImmGen cytokines: Signatures of “common γ-chain” family cytokines

Project description:Profiling of early in-vivo responses to the 6 common gamma-chain family cytokines, in 14 immunologic cell-types.

2022-01-01 | GSE180020 | GEO

ImmGen cytokines: Signatures of “common γ-chain” family cytokines

Project description:ImmGen cytokines: Signatures of “common γ-chain” family cytokines

| PRJNA746283 | ENA

Common gamma chain (γc) cytokines differentially potentiate TNFR family signaling in antigen-activated CD8(+) T cells.

Project description: Not available

| S-EPMC4236884 | biostudies-other

The interweaved signatures of common-gamma-chain cytokines across immunologic lineages

Project description:"γc" cytokines are a family whose receptors share a “common gamma chain” signaling moiety, and play central roles in the differentiation, homeostasis and communications of all immunocyte lineages. As a resource to better understand their range and specificity of action, we profiled by RNAseq the immediate-early responses to the main γc cytokines, across all immunocyte lineages. The results show a different response landscape than expected: broader, with a strong Myc-controlled resetting of biosynthetic and metabolic pathways, a major downregulation component, and extensive overlap between cytokines (one cytokine doing in one cell what another does elsewhere). Various mechanisms appear involved: fast transcriptional activation, chromatin remodeling, and mRNA destabilization. Other surprises are uncovered: IL2 effects in mast cells, major shifts between follicular and marginal-zone B cells, paradoxical and cell-specific cross-talk between IFN and γc signaling, or an NK-T like program induced by IL21 in CD8+ T cells.

2022-07-22 | GSE208138 | GEO

Human CD8+CD28- T suppressor cells expanded by common gamma chain (?c) cytokines retain steady allospecific suppressive capacity in vivo.

Project description: Not available

| S-EPMC7189520 | biostudies-literature

Common gamma chain cytokines in combinatorial immune strategies against cancer.

Project description: Not available

| S-EPMC4710466 | biostudies-literature

Human islets exposed to cytokines IL-1β and IFN-γ

Project description:In the context of T1 Diabetes, pro-inflammatory cytokines IL-1β and IFN-γ are known to contribute to β-cell apoptosis; The measurement of mRNA expression following β-cell exposure to these cytokines gives a picture of the changes in gene expression characterizing the path to β-cell dysfunction and death. Human islets were isolated and exposed (or not) to IL-1β and IFN-γ. The samples were collected at various time points for profiling with Affymetrix arrays. These measurements were performed three times.

2013-12-19 | E-GEOD-53454 | biostudies-arrayexpress

Microbiota-derived short-chain fatty acids promote the memory potential of antigen activated CD8+ T cells

Project description:Microbiota-derived short-chain fatty acids promote the memory potential of antigen activated CD8+ T cells

| PRJNA548059 | ENA