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Transcriptome comparison of pancreatic islets of Urocortin3 null islets and wild type controls.

ABSTRACT: The peptide hormone Urocortin3 (Ucn3) is abundantly expressed by mature beta cells, yet its physiological role is unknown. Transcriptome data from Ucn3 null islets compared to wild type controls reveals selective depletion for delta cell markers and supports a role for Ucn3 as a trigger for somatostatin-mediated negative feedback.

ORGANISM(S): Mus musculus

PROVIDER: GSE58286 | GEO | 2015/05/22

SECONDARY ACCESSION(S): PRJNA251895

REPOSITORIES: GEO

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Transcriptome comparison of pancreatic islets of Urocortin3 null islets and wild type controls.

Project description:The peptide hormone Urocortin3 (Ucn3) is abundantly expressed by mature beta cells, yet its physiological role is unknown. Transcriptome data from Ucn3 null islets compared to wild type controls reveals selective depletion for delta cell markers and supports a role for Ucn3 as a trigger for somatostatin-mediated negative feedback. Isolated islets from Ucn3 knockout animals compared to wild type littermate controls. Islets from three indivual males were used for each genotype, lysed in Trizol for RNA isolation and library construction.

2015-05-22 | E-GEOD-58286 | biostudies-arrayexpress

RNA-seq of Ins+, Ucn3+/- cell populations within developmental mouse islets using MARIS

Project description:The aim of this experiment was to observe the transcriptional profile of individual populations of cells in mouse islets throughout development. MARIS was used to isolate Ins+ islet cells at E18.5 and P13, and further separate Ucn3- and Ucn3+ populations at the transition point P4 and P5.

2015-03-17 | E-MTAB-3418 | biostudies-arrayexpress

Cdh13 null mice isolated islets RNAseq

Project description:The pancreas islets RNA expressions were compared between WT (exon2 floxed) mice and Cdh13 KO (exon2 deleted) mice. The islets were isolated from the mice at 8wks old, 14wks old under normal chow diet, and 14wks old under HFD conditions.

2022-12-09 | GSE186944 | GEO

Purification of pancreatic endocrine subsets from mouse islets

Project description:We developed a FACS-platform for the isolation of highly purified endocrine subsets from mouse islets, based on specific differential expression of CD24 on delta-cells and CD71 on beta-cells.

2020-04-29 | GSE149488 | GEO

Transcriptomic profiling of alpha, beta, and delta cell populations provides new insights into the role of ghrelin in the pancreas

Project description:Intra-islet crosstalk between islet cells is critical in orchestrating the body’s response to changes in blood glucose levels, but is incompletely understood. In this study, we used transgenic mouse lines that allowed the purification and transcriptomic characterisation of alpha, beta, and delta cells, yielding an RNA-sequencing database that can be searched for regulatory proteins which are differentially expressed between cell types. As an illustrative example, we examined the expression of g-protein coupled receptors, and found that the ghrelin receptor, Ghsr, was highly expressed in delta cells compared to alpha and beta cells. GHSR excitation elicited increases in cytosolic calcium levels in primary delta cells. In the perfused pancreas, the application of ghrelin stimulated somatostatin secretion, correlating with a decrease in insulin and glucagon release, which was sensitive to somatostatin receptor antagonism. These results show that ghrelin acts specifically on delta cells within pancreatic islets to affect blood glucose regulation.

2016-07-11 | GSE76017 | GEO

Virgin Beta Cells Persist throughout Life at a Neogenic Niche within Pancreatic Islets

Project description:Beta cells are the sole source of insulin in our body, yet we do not understand how they mature into fully functional, glucose-responsive beta cells. We discovered a persistent population of immature beta cells that is marked by the absence of Ucn3 and is functionally immature. We generated transcriptomes of these immature beta cells, along with mature beta cells and the mixed pool of non-beta cells from the same islets. This enables a systematic comparison across thousands of genes between two distinct maturation stages of the beta cell.

2017-04-04 | GSE88778 | GEO

Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [human_singlecell]

Project description:Pluripotent stem cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment. Here, we demonstrate that transplantation of pluripotent stem cell-derived islets into diabetic nonprimates effectively restored endogenous insulin secretion and improved glycemic control. Single-cell RNA sequencing analysis of S6D2 clusters confirmed the existence of the three major pancreatic endocrine cell populations (β cells, α-like cells and δ-like cells) and their proportions, which altogether accounted for 80%. Importantly, hierarchical clustering of S6D2 hCiPSC-islets, 10 wpt kidney grafts and primary islets showed that the hCiPSC differentiated pancreatic endocrine cells shared similar global gene expression profiles to their native counterparts in primary islets. Single-cell RNA sequencing analysis on PBMCs revealed the potential immune response of recipient macaque to hCiPSC-islets.

2022-02-04 | GSE185036 | GEO

RNA-seq of Ins+, Ucn3+/- cell populations within developmental mouse islets using MARIS

Project description:RNA-seq of Ins+, Ucn3+/- cell populations within developmental mouse islets using MARIS

| PRJEB8849 | ENA

Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [monkey_singlecell]

Project description:Human pluripotent stem cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment. Here, we demonstrate that transplantation of human pluripotent stem cell-derived islets into diabetic nonhuman primates effectively restored endogenous insulin secretion and improved glycemic control. Single-cell RNA sequencing analysis of S6D2 clusters confirmed the existence of the three major pancreatic endocrine cell populations (β cells, α-like cells and δ-like cells) and their proportions, which altogether accounted for 80%. Importantly, hierarchical clustering of S6D2 hCiPSC-islets, 10 wpt kidney grafts and primary human islets showed that the hCiPSC differentiated pancreatic endocrine cells shared similar global gene expression profiles to their native counterparts in primary human islets. Single-cell RNA sequencing analysis on PBMCs revealed the potential immune response of recipient macaque to hCiPSC-islets.

2022-02-04 | GSE185037 | GEO

Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [human_bulk]

2022-02-04 | GSE185035 | GEO

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