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Gene expression in Rainbow trout (Oncorhynchus mykiss) fry gonads after a chronic exposure to a range of 17α-ethynylestradiol concentrations

ABSTRACT: Sex steroids play a key role in triggering sec differentiation in fish and the use of exogenous hormone treatment leads to partial or complete sex reversal. This phenomenon has attracted attention since the discovery taht even low environmental doses of exogenous steroids can adversely affect gonad morphology (ovotestis development) and induce reproductive failure. Modern genomic-based technologies have enhanced opportunities to find mechanisms of action (MOA) and dentify biomarkers for the toxic action of a compound. The goal of this study are to improve the understanding of feminization in fish by analyzing gene expression patterns in the gonads of rainbow trout fry after a chronic exposure to several doses (0.01, 0.1, 1 and 10 μg/L) of ethynylestradiol (EE2) and to offer target genes as potential biomarkers of ovotestis development.

ORGANISM(S): Oncorhynchus mykiss

PROVIDER: GSE58519 | GEO | 2015/06/25

SECONDARY ACCESSION(S): PRJNA252817

REPOSITORIES: GEO

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Gene expression in Rainbow trout (Oncorhynchus mykiss) fry gonads after a chronic exposure to a range of 17?-ethynylestradiol concentrations

Project description:Sex steroids play a key role in triggering sec differentiation in fish and the use of exogenous hormone treatment leads to partial or complete sex reversal. This phenomenon has attracted attention since the discovery taht even low environmental doses of exogenous steroids can adversely affect gonad morphology (ovotestis development) and induce reproductive failure. Modern genomic-based technologies have enhanced opportunities to find mechanisms of action (MOA) and dentify biomarkers for the toxic action of a compound. The goal of this study are to improve the understanding of feminization in fish by analyzing gene expression patterns in the gonads of rainbow trout fry after a chronic exposure to several doses (0.01, 0.1, 1 and 10 ?g/L) of ethynylestradiol (EE2) and to offer target genes as potential biomarkers of ovotestis development. An all-male population of Rainbow trout fry was exposed during 76 days (from 60 to 136 days post-fertilization (dpf)) to five nominal concentrations of 17?-ethynylestradiol (0-solvent control-, 0.01, 0.1, 1 and 10 ?g EE2/L of water), using 3 tanks per condition. In total, 30 samples were analyzed independantly: 6 samples per concentration tested (two samples per tank, three tanks per concentration), each sample being a pool of 10 pairs of gonads.

2015-06-25 | E-GEOD-58519 | biostudies-arrayexpress

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Meta-Analysis of Microarray Data of Rainbow Trout Fry Gonad Differentiation Modulated by Ethynylestradiol.

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17α-ethynylestradiol (EE2) limits the impact of ibuprofen upon respiration by streambed biofilms in a sub-urban stream.

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Project description:Sex steroids effect on gut microbiota

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Gonadectomy-induced transcriptome changes in the ventral cervical spinal cord of male and female rats

Project description:Circulating sex steroids are critical for the development of neuroplasticity in the respiratory motor system. A primary locus of inducible respiratory neuroplasticity is the phrenic motor nucleus, a column of motor neurons in the ventral cervical spinal cord (C3-C5). In specific, exposure to acute intermittent hypoxia (AIH) induces strengthened synaptic connections to phrenic motor neurons and increased motor output; a form of neuroplasticity called phrenic long-term facilitation (pLTF). Gonadectomy (surgical removal of the testes in males and ovaries in females) reduces circulating sex steroids and eliminates AIH-induced phrenic pLTF in both females and males, implicating sex steroids as critical mediators for the development of neuroplasticity. To begin interrogating mechanisms of sex steroid influence on phrenic motor neurons and to determine the effect of gonadectomy on local gene expression, we performed RNA sequencing on ventral cervical spinal cord samples containing the phrenic motor nucleus. Our findings identified sex-specific gene expression in gonadally-intact rats and suggested that gonadectomy caused greater changes to the transcriptomes of male rats compared to females.

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Identification of sex steroid hormones binding proteins in Pseudomonas aeruginosa.

Project description:The project aims to elucidate mechanisms driving P. aeruginosa response to sex steroids hormones. An experiment involving Co-immunoprecipitation combined with Mass spectroscopy was performed to identify P. aeruginosa proteins binding to estradiol and /testosterone.

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Impact of 17α-ethynylestradiol (EE2) on activated sludge microbiome

Project description:This study assessed the impact of 17α-ethynylestradiol (EE2) on activated sludge microbiome

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Fathead minnow ovaries exposed in vivo or in vitro to fadrozole

Project description:Interpreting proteomic and genomic data is a major challenge in predictive ecotoxicology that can be addressed by a systems biology approach. Mathematical modeling provides an organizational platform to consolidate protein dynamics with possible genomic regulation. Here, a model of ovarian steroidogenesis in the fathead minnow (Pimephales promelas) is developed to evaluate possible transcriptional regulation of the steroid production observed in microarray studies. Steroidogenesis, the production of sex steroids, is a highly conserved pathway whose regulation is critical to sexual reproduction. The model was developed from literature sources, integrating key signaling components (G-protein and PKA activation) with their ensuing effect on steroid production. The model properly predicted steroid behavior when fish were exposed to fadrozole, a specific aromatase inhibitor, but failed to predict testosterone and estradiol behavior during depuration. In vivo microarray data implicated three modes of regulation which may account for over-production of steroids during depuration: P450 enzyme up-regulation, inhibin down-regulation, or luteinizing hormone up-regulation. Simulation studies and sensitivity analysis were used to evaluate each of the three modes as possible sources of compensation.

2009-05-13 | GSE15924 | GEO

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