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Human MCF-7 breast cancer cells: control vs. treated with conditioned medium of cancer associated adipose tissue

ABSTRACT: Transcriptional profiling of human MCF-7 breast cancer cells comparing MCF-7 cells treated with control medium (DMEM/F12 + 0,5% BSA) with MCF-7 cells treated with conditioned medium of cancer-associated adipose tissue (CMCAAT) obtained from 2 breast cancer patients. Goal was to determine the effects of CMCAAT treatment on global MCF-7 gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE58574 | GEO | 2015/01/07

SECONDARY ACCESSION(S): PRJNA252929

REPOSITORIES: GEO

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Human MCF-7 breast cancer cells: control vs. treated with conditioned medium of cancer associated adipose tissue

Project description:Transcriptional profiling of human MCF-7 breast cancer cells comparing MCF-7 cells treated with control medium (DMEM/F12 + 0,5% BSA) with MCF-7 cells treated with conditioned medium of cancer-associated adipose tissue (CMCAAT) obtained from 2 breast cancer patients. Goal was to determine the effects of CMCAAT treatment on global MCF-7 gene expression. Two-condition experiment: MCF-7 control vs. CMCAAT treated cells. Biological replicates: 3 control replicates pooled as 1 (LL 1-2-4), 4 CMCAAT replicates (LL 6, LL 8, LL 9, LL 10)

2015-01-07 | E-GEOD-58574 | biostudies-arrayexpress

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Conditioned media from Cancer cell lines regulate mRNAs in differentiated human muscle stem cells

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LC-MSMS of microplasts derived from macrophage conditioned medium treated breast adenocarcinoma MCF-7 cells. Pooja Kamal Melwani, M.Sc., Nishad S, PhD, Badri Narain Pandey, PhD, Radiation Signalling and Cancer Biology Section, Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

Project description:Paracrine signals relayed between heterogenous cell types through small and large extracellular vesicles promote cancer cell growth, invasion and metastasis. Microplasts, also called as cytoplasts or cytokineplasts are large extracellular vesicles (0.5-11µm diameter) originating from migratory cells. Microplasts are known to be associated with invasive phenotypes of cancer cells and promote metastasis. Treatment with macrophage conditioned medium induces significant shedding of microplasts from MCF-7 breast adenocarcinoma cells. To characterise microplasts and to study their potential role in intercellular communication in cancer, we isolated microplasts derived from macrophage conditioned medium treated MCF-7 cells and investigated their protein cargo through LC-MSMS.

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