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RNA sequencing of cultured mouse wild-type E10.25 forelimbs in control or cyclopmaine containing media [SOLiD]

ABSTRACT: We wanted to identify differentially expressed genes in wild-type forelimbs and forelimbs briefly exposed to Shh signaling. E10.25 forelimbs were cultured in control media and media containing cyclopmaine (inhibits the Hedgehog pathway)

ORGANISM(S): Mus musculus

PROVIDER: GSE58664 | GEO | 2015/07/01

SECONDARY ACCESSION(S): PRJNA253127

REPOSITORIES: GEO

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RNA sequencing of cultured mouse wild-type E10.25 forelimbs in control or cyclopmaine containing media [SOLiD]

Project description:We wanted to identify differentially expressed genes in wild-type forelimbs and forelimbs briefly exposed to Shh signaling. E10.25 forelimbs were cultured in control media and media containing cyclopmaine (inhibits the Hedgehog pathway) Generated two wild-type (control) and two cyclopmaine biological replicates for RNA-seq UT-Genome and Analysis Facility

2015-07-01 | E-GEOD-58664 | biostudies-arrayexpress

RNA sequencing of mouse littermate wild-type and Shh null E10.5 forelimbs [Illumina]

Project description:We wanted to identify differentially expressed genes in wild-type and Shh null E10.5 mouse forelimbs

2015-07-01 | GSE58645 | GEO

RNA sequencing of mouse littermate wild-type and Shh null E10.5 forelimbs [Illumina]

Project description:We wanted to identify differentially expressed genes in wild-type and Shh null E10.5 mouse forelimbs Used two wild-type technical replicates and two Shh null technical replicates UT-Genome and Analysis Facility

2015-07-01 | E-GEOD-58645 | biostudies-arrayexpress

Expression data from E11.5 mouse embryonic forelimbs - various mutant conditions or dissected limb domains

2008-09-15 | GSE11063 | GEO

Expression data from E11.5 mouse embryonic forelimbs - various mutant conditions or dissected limb domains

Project description:Sonic hedgehog (Shh) signals via Gli transcription factors to direct digit number and identity in the vertebrate limb. We have characterized the Gli-dependent cis-regulatory network through a combination of whole genome ChIP-on-chip and transcriptional profiling of the developing mouse limb. In this dataset, we include the expression data obtained from dissected mouse forelimbs using a variety of gain- and loss-of-function hedgehog pathway mutants, as well as limbs dissected into responsive (posterior 2/3ds) and non-responsive (anterior 1/3d) Hh tissues. These data are used to obtain 753 genes that are differentially expressed in response to Shh signaling. Keywords: Comparison of genetic samples 28 Total samples were analyzed. We generated the following pairwise comparisons using PowerExpress: Shh<WT; SmoM2>WT; Gli3<WT; Gli3>WT; Ant<Post; Ant>Post. Genes with an FDRM-bM-^IM-$10% and a fold-change M-bM-^IM-%2 were selected. We did not generate pairwise comparisons for a certain combinations with SmoGli3 and Gli3 mutants because data from these arrays contained significant variability. To identify additional genes that were Shh-responsive, we performed the following multiple sample comparisons using an FDRM-bM-^IM-$10% and a posterior probability cutoff of M-bM-^IM-$25%: 1.) Ant<Post and Shh<WT<SmoM2, 2.) Ant<Post and Shh<WT<SmoM2 and Gli3>SmoGli3, 3.) Ant<Post and Shh<WT<SmoM2 and WT>SmoGli3

2008-09-14 | E-GEOD-11063 | biostudies-arrayexpress

Expression data from E12 mouse lumbar motor neuron subsets

2017-03-10 | GSE86478 | GEO

Cryptococcus neoformans reactivation in different media and temperatures

Project description:We wanted to find out the transcriptomic response of growth-arrested Cryptococcus yeast cells when initially placed in environments in which they could reactivate or "wake up", focusing on the difference between a rich microbial media (YPD) and a common cell culture media, RPMI + 10% heat-inactivated fetal calf serum. Please contact Edward.Wallace@ed.ac.uk if you use this data and ask for citation information; we are currently (August 2020) working on a publication.

2020-08-26 | GSE133067 | GEO

Expression data from adult male mouse hypothalamus

Project description:Laser captured regions surrounding the perifornical area (PeFA) in adult male mice that attacked pups; a control outside region (unk); a c-fos positive region (c-fos); an oxytocin positive region (oxt) In this dataset, we include the expression data obtained from dissected mouse forelimbs using a variety of gain- and loss-of-function hedgehog pathway mutants, as well as limbs dissected into responsive (posterior 2/3ds) and non-responsive (anterior 1/3d) Hh tissues. These data are used to obtain 753 genes that are differentially expressed in response to Shh signaling.

2021-06-01 | GSE161507 | GEO

Gene Expression profiles of Bapx1 sorted cells from forelimbs (FL) of wildtype (WT) and Bapx1 null (HOMO) E12.5 mouse embryos

Project description:This study aims to look at gene expresion profiles between wildtype and Bapx1 knockout cells of the forelimbs in a E12.5 mouse embryo. Instead of looking at the whole forelimbs, only cells expressing Bapx1 were sorted by Fluroscent Activated Cell Sorting (FACS) and subjected to expression profiling by microarray.

2015-03-19 | GSE35649 | GEO

Compare gene expression patterns in Chsy-1 wt and ko mouse 13.5 days embryo forelimbs

Project description:Transcriptional profiling of 13.5 day mouse embryo forelimbs. Gene expression comparison done between wild type and Chsy-1 gene knockout mice.

2014-06-01 | GSE29694 | GEO

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