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Expression data from PAO1 and its creBC derivative mutant (PAOΔcreBC)

ABSTRACT: We used microarrays to determine the expression profile of the P. aeruginosa creBC mutant regarding its parental wild type strain PAO1.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE58758 | GEO | 2014/06/25

SECONDARY ACCESSION(S): PRJNA253371

REPOSITORIES: GEO

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Expression data from PAO1 and its creBC derivative mutant (PAOM-NM-^TcreBC)

Project description:We used microarrays to determine the expression profile of the P. aeruginosa creBC mutant regarding its parental wild type strain PAO1. Expression analysis from three independent replicates of PAO1 and its creBC derivative mutant (PAOM-NM-^TcreBC) were performed under basal conditions and with cefoxitin and ceftazidime. cDNA synthesis, fragmentation, labeling and hybridization were performed according to the Affymetrix GeneChip P. aeruginosa genome array expression analysis protocol.

2014-06-25 | E-GEOD-58758 | biostudies-arrayexpress

PAO1 strain exposure to thiazolidinedione (TZD) derivative

Project description:The purpose of this study was to define the TZD effect in Pseudomonas aeruginosa. Transcriptional profiling of Pseudomonas aeruginosa wild-type strain,reference strain PAO1, as control Vs. PAO1 strain exposed to a final 0.02mM of TZD derivative ((z)-5-octylidenethiazolidine-2,4-dione).

2014-12-03 | E-MTAB-3144 | biostudies-arrayexpress

Microarray analysis of P. aeruginosa PAO1 expression PA5060 (phaF) on pJN105

2013-06-21 | GSE47173 | GEO

P. aeruginosa PAO1 PA2663 mutant vs. wild type strain

2008-02-22 | GSE9255 | GEO

Microarray analysis of P. aeruginosa PAO1 expression PA5060 (phaF) on pJN105

Project description:The Pseudomonas aeruginosa PAO1 gene phaF (PA5060) is a transcriptional regulator in the closely related pseudomonad P. putida. phaF is expressed at higher levels in P. aeruginosa clinical isolates from the cystic fibrosis respiratory tract. To determine the role of phaF in regulating P. aeruginosa gene expression, we cloned it under control of the pBAD promoter in expression vector pJN105 and compared expression in this strain relative to an empty vector control strain. We used microarrays to study overall gene expression in a P. aeruginosa PAO1 phaF overexpression strain. A P. aeruginosa PAO1 strain over-expressing the transcriptional regulator phaF, PAO1 pJN105-phaF, and the corresponding empty control strain, P. aeruginosa PAO1 pJN105, were grown to exponential phase, RNA was isolated, and gene expression was analyzed via microarray analysis and compared.

2013-06-21 | E-GEOD-47173 | biostudies-arrayexpress

Expression data from Pseudomonas aeruginosa PAO1 and an isogenic HCN negative mutant (PAO6344), grown under cyanogenic conditions.

Project description:Pseudomonas aeruginosa produces the toxic secondary metabolite hydrogen cyanide (HCN) at high cell population densities and low aeration. We have used Affimetrix microarrays to investigate the impact of HCN as a signal in cell-cell communication by comparing the transcriptome of the wild-type strain PAO1 to that of an HCN-negative mutant (PAO6344) under cyanogenic conditions.

2013-09-16 | GSE48587 | GEO

Transcription profiling of P. aeruginosa PAO1 PA2663 mutant vs. wild type strain

Project description:P. aeruginosa PAO1 wild type and PA2663 mutant strains expression in biofilm cells relative to P. aeruginosa PAO1 wild type strain expression in biofilm cells. All samples cultured in LB with glass wool Experiment Overall Design: Strains: P. aeruginosa PAO1 wild-type, PA2663 mutant Experiment Overall Design: Medium: LB Experiment Overall Design: Biofilm grown on glass wool Experiment Overall Design: Time: 24 h Experiment Overall Design: Cell Type: biofilm

2008-04-07 | E-GEOD-9255 | biostudies-arrayexpress

PAO1 gene expression at 22°C and 37°C in Lysogeny broth

Project description:Pseudomonas aeruginosa strain PAO1 was grown at 22°C and 37°C in Lysogeny broth (LB) and RNA was hybridized on the Affymetrix P. aeruginosa chip.

2014-01-01 | GSE51409 | GEO

PAHM4 vs PAO1 37C LB

Project description:Pseudomonas aeruginosa strains PAHM4 and PAO1 were grown at 37C on LB and RNA was hybridized on the Affymetrix P. aeruginosa chip to compare transcript differences from a BQ isolate to a well characterized wound isolate. Strains PAHM4 and PAO1 were grown in triplicate. Total RNA from each sample was pooled and amplified then assayed in triplicate resulting in 6 total samples. PAO1 is the control strain while PAHM4 is the experimental strain.

2012-10-01 | E-GEOD-40461 | biostudies-arrayexpress

Gene expression in PAO1, clonal AES-1 and non-clonal isolates of Pseudomonas aeruginosa

Project description:Pseudomonas aeruginosa (P. aeruginosa) lung infection is a significant cause of mortality in patients with cystic fibrosis (CF). Most CF patients acquire unique P. aeruginosa strains from the environment; however clonal strains have been identified in CF communities in several countries. Two clonal strains infect 10% to 40% of patients in three CF clinics in mainland eastern Australia. The expression profiles of four planktonically-grown isolates of one Australian clonal strain (AES-1), and four non–clonal CF P. aeruginosa isolates were compared to each other and to the reference strain PAO1 using the Affymetrix P. aeruginosa PAO1 genome array, to gain insight into properties mediating the enhanced infectivity of AES-1. The isolates were subsequently grown as 3-day old biofilms and similarly extracted for RNA and compared as above. Data analysis was carried out using BIOCONDUCTOR software. Keywords: Comparative strain hybridization

2008-12-05 | GSE6122 | GEO

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