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Rapid transcriptional response to physiological neuronal activity in vivo revealed by transcriptome sequencing

ABSTRACT: mRNA-seq of olfactory bulbs from odor-deprived and odor-stimulated mice (RNA-seq done in triplicates)

ORGANISM(S): Mus musculus

PROVIDER: GSE58843 | GEO | 2014/06/27

SECONDARY ACCESSION(S): PRJNA253678

REPOSITORIES: GEO

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Rapid transcriptional response to physiological neuronal activity in vivo revealed by transcriptome sequencing

Project description:mRNA-seq of olfactory bulbs from odor-deprived and odor-stimulated mice (RNA-seq done in triplicates) Control mice were kept in a clean air environment (using an activated carbon filter). Stimulated mice were exposed to a cocktail of odorants for 30 minutes ("30 min" time point), or for 30 minutes followed by a period of 90 minutes of clean air ("120 min" time point.) Mice were sacrificed immediately at end of time point, and olfactory bulbs were dissected out for RNA extraction and RNA-FISH. Three adult mice (6-8 weeks of age or older) were used in each condition.

2014-06-27 | E-GEOD-58843 | biostudies-arrayexpress

Induction of astrocytic Slc22a3 regulates sensory processing through histone serotonylation (ChIP-Seq)

Project description:To profile Sox2 and Sox9 binding sites in Gq-DREADD expressing CNO treated brains in comparison to control. Furthermore, we also aimed to profile Sox9 binding sites in odor treated vs. control olfactory bulbs. An additional goal of this study was to profile histone serotonylation mark in Slc22a3-cKO olfactory bulb in comparison to control.

2023-04-17 | GSE229644 | GEO

Transcription profiling of murine olfactory epithelium during neuronal death and replacement to study adult neurogenesis in the olfactory epithelium

Project description:Expression profiling of mRNA abundance in the adult mouse olfactory epithelium during replacement of OSNs forced by the bilateral ablation of the olfactory bulbs. The experiment was done on 6 week old male C57Bl/6 mice. Olfactory epithelium tissue samples were collected on days 1, 5, and 7 after bulbectomy. The cellular processes activated by bulbectomy include apoptosis of mature olfactory sensory neurons, infiltration of macrophages and dendritic cells, stimulation of proliferation of basal cell progenitors, and differentation of new sensory neurons.

2007-09-04 | E-GEOD-2437 | biostudies-arrayexpress

Transcriptional changes during neuronal death and replacement in the adult olfactory epithelium

2005-08-01 | GSE2437 | GEO

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (scRNA-Seq)

Project description:A reduced sense of smell has been reported in people with cystic fibrosis (CF). These olfactory defects have largely been attributed to secondary manifestations of the disease, such as inflammation of the nasal mucosa. Here we show that CFTR, the gene responsible for CF, is expressed in proliferating olfactory human cells and that newborn CFTR null pigs display ultrastructural abnormalities in the olfactory epithelium and olfactory bulbs. In the absence of CFTR, olfactory sensory neurons still produce odor-evoked activity, but mutant animals display defective odor-guided suckling behavior after birth. Consistent with epithelial changes, we found a reduced expression of genes implicated in cell cycle and development in globose basal cells (GBCs), the neurogenic progenitor cells in the olfactory epithelium. Targeted sequencing revealed enhanced CFTR expression in the subpopulation of GBCs that is actively proliferating. Furthermore, CFTR loss caused a global reduction in the number of sensory neurons and altered olfactory receptors expression. Our findings highlight a previously unknown role of CFTR in olfactory system function by regulating progenitor cell proliferation in the olfactory epithelium.

2022-12-31 | GSE197189 | GEO

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (Bulk RNA-Seq)

2022-12-31 | GSE197184 | GEO

Transcription factors expressed in olfactory bulb local progenitor cells revealed by genome-wide transcriptome profiling

Project description:We used transcriptional profiling to monitor gene expression of whole olfactory bulbs at daily intervals from embryonic day 11 through birth, generating a compendium of gene expression encompassing the major developmental events of this tissue.

2011-01-04 | GSE26406 | GEO

Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo

Project description:We present a high-throughput in vivo method to identify odorant receptors responding to odorants, using phosphorylated ribosome immunoprecipitation of mRNA from olfactory epithelium of odor-stimulated mice followed by RNA-Seq. pS6-IP RNA-Seq in odor stimulated vs control mice olfactory epithelium

2015-08-24 | E-GEOD-59324 | biostudies-arrayexpress

Olfactory proteomics in Dementia with Lewy bodies

Project description:Proteomic analysis were performed in olfactory bulbs derived from controls and individuals with dementia with lewy bodies

2021-09-08 | PXD016069 | Pride

Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia (scRNA-seq: olfactory bulb)

Project description:A subset of COVID-19 patients exhibit altered olfactory function. Here we analyze bulk and single cell RNA-Seq datasets to identify cell types in the olfactory epithelium and olfactory bulb that express cell entry molecules that mediate infection by SARS-CoV-2 (CoV-2), the causal agent in COVID-19. We find that samples from whole olfactory mucosa in species including mouse and human express two key genes involved in CoV-2 entry, ACE2 and TMPRSS2. However, neither olfactory sensory neurons nor olfactory bulb neurons express these genes, which are instead expressed in support cells, stem cells, and perivascular cells. These findings suggest that CoV-2 infection of non-neuronal cell types leads to anosmia and related disturbances in odor perception in COVID-19 patients.

2020-07-30 | GSE148360 | GEO

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