Dataset Information


Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 5% O2 levels

ABSTRACT: Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity. Keywords: time course Overall design: G-CSF mobilized peripheral blood CD34-positive cells were cultured with IL-3, IL-6, SCF and G-CSF under 5% O2. Three biological replicate experiments were analyzed and approximately 15% of the samples were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.

INSTRUMENT(S): Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version)

ORGANISM(S): Homo sapiens  

SUBMITTER: Eleftherios Terry Papoutsakis  

PROVIDER: GSE5918 | GEO | 2007-06-06



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