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Generating IGF1 signatures in human melanoma cell lines by activation of IGF1 or ihibition of IGF1R

ABSTRACT: To generate IGF1 signatures in human melanoma cell lines. Recombinant human IGF1 (rhIGF1) was added to WM35, known to be responsive to rhIGF1, whereas constitutive IGF1 signaling in Mel-624 was abrogated by siRNA directed against the IGF1 receptor (siIGF1R). Experiments were performed in triplicate in normoxia (O2 concentration, 21%) and hypoxia (1% O2), because hypoxia has been suspected to play an adverse role in melanoma. After generating GEP data, we created signatures of genes significantly (p ≤0.0001, q ≤0.05) and consistently upregulated in rhIGF1-treated WM35 cells, or similarly downregulated in siIGF1R-treated Mel-624 cells, as compared to control cells at the same O2 concentration.

ORGANISM(S): Homo sapiens

PROVIDER: GSE59343 | GEO | 2018/01/01

SECONDARY ACCESSION(S): PRJNA255116

REPOSITORIES: GEO

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