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Expression data from MCF7 breast epithelial cells in normoxic and hypoxic conditions.

ABSTRACT: G9a is able to silence gene expression in hypoxic condition by increasing histone H3K9me2. We have identified a set of genes that are negatively regulated by G9a in hypoxia-dependent manner. In this dataset, we include the expression data obtained from MCF7 breast epithelial cells that have been transfected with control (WT) or G9a shRNAs (KD) and exposed to either normoxia or hypoxia. These data are used to obtain 829 genes that are differentially expressed in response to hypoxia, and 205 genes that are sentisive to G9a level.

ORGANISM(S): Homo sapiens

PROVIDER: GSE59449 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA255340

REPOSITORIES: GEO

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Expression data from MCF7 breast epithelial cells in normoxic and hypoxic conditions.

Project description:G9a is able to silence gene expression in hypoxic condition by increasing histone H3K9me2. We have identified a set of genes that are negatively regulated by G9a in hypoxia-dependent manner. In this dataset, we include the expression data obtained from MCF7 breast epithelial cells that have been transfected with control (WT) or G9a shRNAs (KD) and exposed to either normoxia or hypoxia. These data are used to obtain 829 genes that are differentially expressed in response to hypoxia, and 205 genes that are sentisive to G9a level. 4 samples were analysed. We generated comparisons between WT and KD in normoxic as well as hypoxic condition. Genes differentially expressed in hypoxic condition were selected followed by selection of genes that lose this differential expression upon G9a knockdown.

2015-12-31 | E-GEOD-59449 | biostudies-arrayexpress

RNA-seq of HeLa cells stably overexpressing SRSF6-GFP grown under normoxic and hypoxic conditions

Project description:We used HeLa cells that overexpress GFP-tagged SRSF6 (4-fold) were grown in normal conditions (Normoxia, 21% oxygen) or hypoxic conditions (Hypoxia, 0.2% oxygen). compared the binding pattern of SRSF6 between normoxia, 4h hypoxia and 24h hypoxia.

2023-01-03 | GSE198308 | GEO

RNA sequencing data for hypoxic and normoxic vessels

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of mouse pulmonary arteries (main, right and left extralobar branches) from C57BL/6 mice exposed to 6 weeks or hypoxia (FiO2 10%) and normoxia (FiO2%)

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Analysis of gene expression in hypoxic and normoxic HeLa cells.

Project description:The purpose of experiment is to compare the mRNA expression differences between HeLa cells conditioned with normoxia 21% oxygen and hypoxia 1% oxygen at 6h and 20h.

2016-03-11 | GSE79069 | GEO

Impact of NLUCAT1 deletion on A549 cells in normoxic and hypoxic conditions

Project description:Three independent parental or NLUCAT1 CRISPR/Cas9-deleted A549 clones were cultured in normoxia (Nx) or exposed to hypoxia (Hx) at 1% O2 for 24 hours.

2019-09-04 | GSE117036 | GEO

Transcriptome-wide mapping of RNA:protein interactions of SRSF6 in HeLa cells grown under normoxic and hypoxic conditions by iCLIP

Project description:We used HeLa cells that express GFP-tagged SRSF6 at physiological levels and subjected them to 4 h or 24 h hypoxia (0.2% oxygen). Cells grown in normal conditions were used as control (Normoxia, 21% oxygen). We performed iCLIP using anti-GFP antibodies and compared the binding pattern of SRSF6 between normoxia, 4h hypoxia and 24h hypoxia.

2023-01-03 | GSE198309 | GEO

Profile of mRNAs associated with Ago (Argonaute) 1 in normoxic and hypoxic endothelial cells

Project description:Messenger RNAs that are bound to Ago1 is to be transcriptionally or translational suppressed. Therefore the changes in Ago1-associated transcripts indicate the changes of suppression of these genes. We used microarrays to detail changes of mRNA transcripts that are associated with Ago1 in endothelial cells under normoxia and hypoxia conditions. Human umbilical vein endothelial cells pooled from 8 different sources were subjected to normoxia (21% oxygen, Nx) or hypoxia (2% hypoxia, Hx) for 24 hr. Ago1 was immunoprecipitated with mouse anti-human Ago1 using a lysis buffer containing 0.1% NP-40. The associated RNA was extracted and hybridized on Affymetrix Human Gene 1.0 ST arrays.

2013-06-30 | E-GEOD-43165 | biostudies-arrayexpress

Analysis of gene expression in hypoxic and normoxic HeLa cells.

Project description:The purpose of experiment is to compare the mRNA expression differences between HeLa cells conditioned with normoxia 21% oxygen and hypoxia 1% oxygen at 6h and 20h. Total RNA was obtained from HeLa cells for 4 different conditions, 3 biological replicate experiments were performed, resulting in a total of 12 samples.

2016-03-11 | E-GEOD-79069 | biostudies-arrayexpress

Profile of mRNAs associated with Ago (Argonaute) 1 in normoxic and hypoxic endothelial cells

2013-06-30 | GSE43165 | GEO

lncRNA expression profiles in OSCC cells cultured under normoxic and hypoxic conditions

Project description:Hypoxic microenvironment plays important roles in the progression of solid tumors including oral squamous cell carcinoma (OSCC). Long noncoding RNAs (lncRNAs) have gained much attention in the past few years. However, it is not clear whether lncRNAs could regulate hypoxia-adaptation of OSCC, and which lncRNAs participate in this process. Using an lncRNA microarray, we analyzed the aberrant lncRNA expression profiles in OSCC tissues compared with paired normal oral mucosa as well as in hypoxic OSCC cells compared with normoxic OSCC cells. Our microarray also identified 942 lncRNAs that were up-regulated and 507 lncRNAs that were down-regulated in cells cultured under hypoxia compared with those cultured under normoxia (Fold Change >= 2.0, P-value <= 0.05).

2016-07-26 | GSE84790 | GEO

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