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Autophagy mediates the mitotic senescence transition

ABSTRACT: ER:RAS-G12V expressing IMR90 cells were given 100nM 4-OHT and assessed for transcriptional profiling at various time points.

ORGANISM(S): Homo sapiens

PROVIDER: GSE59522 | GEO | 2014/07/31

SECONDARY ACCESSION(S): PRJNA255498

REPOSITORIES: GEO

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Autophagy mediates the mitotic senescence transition

Project description:ER:RAS-G12V expressing IMR90 cells were given 100nM 4-OHT and assessed for transcriptional profiling at various time points. IMR90 cells expressing a 4-hydroxytamoxifen (4-OHT) inducible estrogen receptor (ER)-coupled RAS-G12V (ER:RAS-G12V) were given 100nM 4-OHT for these eight time points (0, 2, 8, 24hrs, 2, 4, 6 and 8days) then analysed for transcriptional profiling using the illumina expression array platform (human-6 v2 Expression BeadChips). There were three biological replicates (batch A, B and D) for each of the time points.

2014-07-31 | E-GEOD-59522 | biostudies-arrayexpress

NOTCH1 mediates a reciprocal switch between two distinct secretomes during senescence [N1ICD]

Project description:ER:RAS-G12V expressing IMR90 cells were transduced with N1ICD-containing or control vectors before treatment with either 100nM 4-OHT or vehicle for 6 days leading to Notch-induced senescence (NIS), RAS-induced senescence (RIS) or combined Notch and Ras-induced senescence (RNIS).

2016-06-22 | GSE72404 | GEO

NOTCH1 mediates a reciprocal switch between two distinct secretomes during senescence [CSM]

Project description:ER:RAS-G12V expressing IMR90 cells were treated with either 100nM 4-OHT for 6 days or 100uM Etoposide for 2 days, followed by further culture for 5 days, leading to RAS-induced senescence (RIS) and DNA-damage induced senescence (DDIS) respectively.

2016-06-22 | GSE72407 | GEO

The effect of COX2 inhibition on RAS-induced senescence

Project description:ER:RAS-G12V expressing IMR90 cells were treated with either 100nM 4-OHT or 40uM Celecoxib or both for 6 days leading to RAS-induced senescence (RIS) with or without COX2 inhibition.

2021-01-25 | GSE145650 | GEO

Effect of NMTi on senescence model systems

Project description:To examine effects of NMT inhibition on senescence transcriptional signatures we treated IMR90 ER:RAS cells with either IMP1088 (300nM) or DDD86481 (1.5uM) for 72h 7 days after induction with 4-OHT treatment. Transcriptional analysis was performed 3 days after drug treatment and 10 days after senescence induction.

2023-09-28 | GSE224071 | GEO

Expression data from paracrine senescence

Project description:Senescence can be transmitted in a paracrine way from cells undergoing Oncogene Induced Senescence (OIS) to naM-CM-/ve normal cells. We define this phenomenon as M-bM-^@M-^\paracrine senescenceM-bM-^@M-^] We used microarrays to compare the trancriptome of cells undergoing paracrine senescence to the transcriptome of cells suffering OIS to unveil the common signatures defining both events and the similarities between them IMR90 cells were co-cultured with IMR90 ER:RAS undergoing OIS or IMR90-Vector control cells using 0.2 M-NM-<m pore transwell (anopore) to allow communication of soluble factors but physical separation of the two cell populations. The total mRNA of IMR90, IMR90 ER:RAS or IMR90 cells cultured in Transwells together with IMR90 vector or IMR90 ER:RAS cells during 7 days in the presence of 200 nM 4OHT and 0.5 % FBS was extracted and hybridized on Affymetrix microarrays to compare paracrine senescence to OIS.

2013-06-13 | E-GEOD-41318 | biostudies-arrayexpress

Expression data from ER32 and ER32.40HT IMR90

Project description:IMR90 cells were infected with pLNC-RAS:ER (from Jesus Gil lab) with retroviral gene transfer. Infected cells were drug selected G418. The cells were induced either with ethanol as control or with 100nM final conc 4-hydroxytamoxifen (sigma H7904) for ectopic expression of protein

2014-11-26 | GSE52848 | GEO

Gene expression profiles of Ras mutants.

2009-02-21 | GSE14934 | GEO

Analysis of senescence transcriptome upon knockdown of XPO7 [RNA-seq]

Project description:We depleted XPO7 by shRNA in IMR90 ER:RAS cells. 5 days after RAS induction by treatment with 4OHT we collected RNA. XPO7 depletion alleviates senescence induction.

2021-02-12 | GSE153921 | GEO

Gene expression profiles of Ras mutants.

Project description:Gene expression profiles were collected from HEK-HT cells expressing H-Ras with Ras-activating (G12V), Raf-activating (G12V,T35S), RalGEF-activating (G12V,E37G), or PI3K-activating (G12V,Y40C) mutations. Keywords: Comparison of Ras pathway components. THX=control; 12V=G12V mutation; 35S=G12V,T35S mutation; 37G=G12V,E37G mutation; 40C=G12V,Y40C mutation. 5 replicates of each. Total of 25 samples.

2009-02-21 | E-GEOD-14934 | biostudies-arrayexpress

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