MRNA expression in human DAOY cells

ABSTRACT: We generate ZNF423 knockdown and control DAOY cells with lentivirus that co-expressed the fluorescent protein mCherry. We performed whole genome RNA sequencing (RNA-seq) of three batched of cultured ZNF423 KD or control KD cells. The sequence reads were analyzed by Homer followed by edgeR. The analyzed RNA-seq results showed differential expression profile including 12 known cilia genes, and 3 of these were validated with qRT-PCR on mouse granule cell precursors. This study proved data how ZNF423 linked to cilia complexes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE59598 | GEO | 2016/12/30

SECONDARY ACCESSION(S): PRJNA255674

REPOSITORIES: GEO