A Non-Canonical Nuclear Activity Triggered by Small RNAs and Argonaute Proteins in Human Cells

ABSTRACT: In this study we report the discovery of an unexpected nuclear activity of small RNAs that target an ~8.6 megabase chromosomal domain located on the telomeric q-arm of chromosome 5 from an unbiased genome-wide RNAi screen. Short-hairpin RNAs that target genes within this large domain induce trans-activation of CREB signaling transcriptional reporters and induce an endogenous gene expression signature that includes CREB-target and neuronal-fate genes. Furthermore, CRISPR-guided deletion of AGO-1 and -2 demonstrate their mutually redundant roles in this mechanism, which we named Chromosomally Induced Trans Activation by RNAi (CITAR).

ORGANISM(S): Homo sapiens

PROVIDER: GSE59621 | GEO | 2015/07/19

SECONDARY ACCESSION(S): PRJNA255768

REPOSITORIES: GEO