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Transcriptome maps of mRNP biogenesis factors define pre-mRNA recognition

ABSTRACT: Biogenesis of eukaryotic messenger ribonucleoprotein complexes (mRNPs) involves the synthesis, splicing, and 3’-processing of pre-mRNA, and the assembly of mature mRNPs for nuclear export. We mapped 23 mRNP biogenesis factors onto the newly synthesized yeast transcriptome, providing ~10^5-10^6 high-confidence RNA interaction sites per factor.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE59676 | GEO | 2014/07/23

SECONDARY ACCESSION(S): PRJNA255917

REPOSITORIES: GEO

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Transcriptome maps of mRNP biogenesis factors define pre-mRNA recognition

Project description:Biogenesis of eukaryotic messenger ribonucleoprotein complexes (mRNPs) involves the synthesis, splicing, and 3M-bM-^@M-^Y-processing of pre-mRNA, and the assembly of mature mRNPs for nuclear export. We mapped 23 mRNP biogenesis factors onto the newly synthesized yeast transcriptome, providing ~10^5-10^6 high-confidence RNA interaction sites per factor. PAR-CLIP data of 23 mRNP biogenesis factors in Saccharomyces cerevisiae

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Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA

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The SR-like protein Npl3 transfers mRNP components from the transcription site onto the mRNA

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