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Vibrio vulnificus : biofilm cells vs. planktonic cells

ABSTRACT: The profiles of transcripts from the planktonic cells and biofilm cells of V. vulnificus were compared by using a V. vulnificus whole-genome microarray

ORGANISM(S): Vibrio vulnificus CMCP6 Vibrio vulnificus

PROVIDER: GSE59753 | GEO | 2014/07/26

SECONDARY ACCESSION(S): PRJNA256172

REPOSITORIES: GEO

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Vibrio vulnificus : biofilm cells vs. planktonic cells

Project description:The profiles of transcripts from the planktonic cells and biofilm cells of V. vulnificus were compared by using a V. vulnificus whole-genome microarray Two-condition experiment, planktonic cells vs. biofilm cells. Biological replicates: 3 control, 3 experimental, independently grown and harvested. One replicate per array. For transcriptome analysis, the V. vulnificus whole genome TwinChip, manufactured and kindly provided by the 21C Frontier Microbial Genomics and Applications Center (Daejeon, South Korea), was used.

2014-07-26 | E-GEOD-59753 | biostudies-arrayexpress

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Vibrio vulnificus

Project description:Vibrio vulnificus : biofilm cells vs. planktonic cells

| PRJNA256172 | ENA

Vibrio vulnificus: Wild type vs. iscR mutant (V. vulnificus iron-sulfur cluster regulator)

Project description:IscR is a novel global regulator potentially contributing to the overall success in survival and pathogenesis of V. vulnificus by coordinating the regulation of various virulence factors. The profiles of transcripts from the V. vulnificus iscR mutant and the parental wild type were compared by using a V. vulnificus whole-genome microarray. Two-condition experiment: Wild type vs. iscR mutant. Biological replicates: 3 control, 3 mutant strains, independently grown and harvested. One replicate per array. For transcriptome analysis, the V. vulnificus whole genome TwinChip, manufactured and kindly provided by the 21C Frontier Microbial Genomics and Applications Center (Daejeon, South Korea), was used.

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Vibrio vulnificus: normal intracellular c-di-GMP level vs. high intracellular c-di-GMP level

Project description:The profiles of transcripts from the V. vulnificus grown under normal and high c-di-GMP conditions were compared by using a V. vulnificus whole-genome microarray

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Haemophilus parasuis F9 transcriptome in biofilm and planktonic cultures using RNA sequencing

Project description:Purpose: Study transcriptome differences between biofilm, planktonic and stationary cultures. Methods: Total mRNA from in vitro cultures was extracted and sequenced using Ion Torrent PGM sequencer. Results: Characteristic transcriptomic profile was observed for biofilm, planktonic and stationary cultures. Biofilm and planktonic were similar biological states. Conclusions: Results suggest that H. parasuis F9 has more active metabolism during biofilm or planktonic growth when compared to stationary culture. Some identified membrane-related genes could play an important role in biofilm life.

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