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Gene expression profiling of Lgr5-creERT2/PIK3CA H1047R and K8-creERT2/PIK3CA H1047R-evoked mammary tumors

ABSTRACT: This study examined the gene expression profile of mammary tumors derived from Lgr5- and K8-positive cell-of-origins

ORGANISM(S): Mus musculus

PROVIDER: GSE59872 | GEO | 2015/08/12

SECONDARY ACCESSION(S): PRJNA256962

REPOSITORIES: GEO

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Gene expression profiling of Lgr5-creERT2/PIK3CA H1047R and K8-creERT2/PIK3CA H1047R-evoked mammary tumors

Project description:This study examined the gene expression profile of mammary tumors derived from Lgr5- and K8-positive cell-of-origins Mammary tumors and reference mammary glands from control mice were collected, cryo-homogenized, total RNA was isolated and analyzed by microarray (total 25 samples).

2015-08-12 | E-GEOD-59872 | biostudies-arrayexpress

Gene expression profiling of preneoplastic K8-creERT2/PIK3CAH1047R mammary subsets

Project description:This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from K8-creERT2/PIK3CA H1047R mice Mammary cell subpopulations were isolated from K8-creERT2/PIK3CAH1047R and K8-creERT2 control animals 4 weeks after activation of PIK3CA H1047R transgene expression by Tamoxifen injection. Pooled mammary glands of 2-3 estrus-synchronized mice per genotype were sorted in 3 independent sortings and used for microarray analysis (20 samples in total).

2015-08-12 | E-GEOD-65411 | biostudies-arrayexpress

Gene expression profiling of preneoplastic K8-creERT2/PIK3CAH1047R mammary subsets

Project description:This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from K8-creERT2/PIK3CA H1047R mice

2015-08-12 | GSE65411 | GEO

PIK3CA(H1047R)-evoked breast tumorigenesis

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-08-12 | E-GEOD-59874 | biostudies-arrayexpress

Gene expression profiling of preneoplastic Lgr5-creERT2/PIK3CAH1047R mammary subsets

Project description:This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from Lgr5-creERT2/PIK3CA H1047R mice Mammary cell subpopulations were isolated from Lgr5-creERT2/PIK3CA H1047R and Lgr5-creERT2 control animals 4 weeks after activation of PIK3CA H1047R transgene expression by Tamoxifen injection. Pooled mammary glands of 2-3 estrus-synchronized mice per genotype were sorted in 3 independent sortings and used for microarray analysis (24 samples in total).

2015-08-12 | E-GEOD-59870 | biostudies-arrayexpress

PIK3CA(H1047R)-evoked breast tumorigenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-08-12 | GSE59874 | GEO

Gene expression profiling of preneoplastic Lgr5-creERT2/PIK3CAH1047R mammary subsets

Project description:This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from Lgr5-creERT2/PIK3CA H1047R mice

2015-08-12 | GSE59870 | GEO

Gene expression profiling in mice overexpressing human wild type (WT) keratin 8 (K8) or K8 variants (G62C and R341H).

Project description:3 months old untreated male/female animals were used in the experiment. We used microarray analysis to determine differential gene expression in mice livers overexpressing WT K8 and K8 variants. Mice liver tissues were used for RNA extraction and hybridization on Affymetrix microarrays.

2014-08-27 | E-GEOD-60780 | biostudies-arrayexpress

RNA-Seq data for Trp53/Brca1-null premalignant mammary epithelial cells with a K8+ luminal origin

Project description:BRCA1 mutation-carriers are predisposed to develop Basal-like breast cancer (BLBC), and p53 mutations are present in the majority of human BLBC cases, suggesting loss of these two tumor suppressors play key roles in development of BLBC. Recent studies suggest that the majority of human breast cancers, including BLBC, may originate from mammary epithelial cells (MECs) in the luminal lineage. However, how loss of p53 and BRCA1 contributes to development of BLBC from luminal MECs remains largely elusive. We developed a novel genetic targeting and lineage tracing approach based on intraductal injection of Cre-expressing adenovirus under the control of the pan-luminal Keratin 8 (K8) promoter (Ad-K8-Cre). We performed intraductal injection of Ad-K8-Cre to female mice carrying conditional knockout alleles of Brca1 (Brca1L) and Trp53 (Trp53L). The injected females developed mammary tumors similar to human BLBC within 12 months after injection. Here we characterized MECs targeted by Ad-K8-Cre one month after the intraductal injection.

2019-06-27 | GSE126761 | GEO

Gene expression profiling in mice overexpressing human wild type (WT) keratin 8 (K8) or K8 variants (G62C and R341H).

2014-08-27 | GSE60780 | GEO

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