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Foxo3 Regulated Genes in G1E-ER-GATA-1 Cells

ABSTRACT: The ensemble of Foxo3-regulated genes in the erythroid G1E-ER-GATA-1 cell line was determined by knocking down Foxo3 using siRNA, and measuring genome wide transcription by microarray analysis

ORGANISM(S): Mus musculus

PROVIDER: GSE60137 | GEO | 2014/08/09

SECONDARY ACCESSION(S): PRJNA257835

REPOSITORIES: GEO

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Foxo3 Regulated Genes in G1E-ER-GATA-1 Cells

Project description:The ensemble of Foxo3-regulated genes in the erythroid G1E-ER-GATA-1 cell line was determined by knocking down Foxo3 using siRNA, and measuring genome wide transcription by microarray analysis G1E-ER-GATA-1 cells were treated with control or Foxo3-specific siRNA by nucleofection at t = 0 h and t = 24 h. At t = 24 h, cells were treated with M-CM-^_-estradiol to activate ER-GATA-1. RNA was harvested at t = 48 h and processed for microarray analysis.

2014-08-09 | E-GEOD-60137 | biostudies-arrayexpress

Expression data from ETO2/LMO2 knockdown G1E-ER-GATA-1 cells

Project description:This SuperSeries is composed of the following subset Series: GSE24333: Expression data from ETO2 knockdown G1E-ER-GATA-1 cells GSE24336: Expression data from LMO2 knockdown G1E-ER-GATA-1 cells Refer to individual Series

2010-09-25 | E-GEOD-24359 | biostudies-arrayexpress

Mi2ß knockdown in ß-estradiol-treated (24 h) G1E-ER-GATA-1 cells

Project description:Objective: To determine the extent to which GATA-1 utilizes Mi2ß to regulate gene transcription in the context of G1E-ER-GATA-1 cells.

2013-07-04 | GSE48188 | GEO

Expression data from LMO2 knockdown G1E-ER-GATA-1 cells

Project description:We used microarrays to examine what genes could be regurated by LMO2 in erythroid cells. Comparing expression profile in murine G1E-ER-GATA-1 cells treated with control and Lmo2 siRNA on Agilent array. After siRNA transfection, the cells were treated with b-estradiol for 24h to induce GATA-1-mediated erythroid maturation.

2010-09-25 | E-GEOD-24336 | biostudies-arrayexpress

Expression data from ETO2 knockdown G1E-ER-GATA-1 cells

Project description:We used microarrays to examine what genes could be regulated by ETO2 in erythroid cells. Comparing expression profile in murine G1E-ER-GATA-1 cells treated with control and ETO2(Cbfa2t3) siRNA on Agilent array. After siRNA transfection, the cells were treated with b-estradiol for 24h to induce GATA-1-mediated erythroid maturation.

2010-09-25 | E-GEOD-24333 | biostudies-arrayexpress

Transcription profiling of mouse FOG-1-binding defective mutant of GATA-1, ER-GATA-1(V205G), was expressed in GATA-1-null G1E cells

Project description:Identification of genes regulated by GATA-1 independent of the cofactor FOG-1. Experiment Overall Design: A conditionally activated FOG-1-binding defective mutant of GATA-1, ER-GATA-1(V205G), was expressed in GATA-1-null G1E cells. Transcipt levels were compared in cells untreated or treated with estradiol to activate the GATA-1 mutant.

2008-06-15 | E-GEOD-6958 | biostudies-arrayexpress

Mi2M-CM-^_ knockdown in M-CM-^_-estradiol-treated (24 h) G1E-ER-GATA-1 cells

Project description:Objective: To determine the extent to which GATA-1 utilizes Mi2M-CM-^_ to regulate gene transcription in the context of G1E-ER-GATA-1 cells. 3 Mi2M-CM-^_ knockdown samples were compared to 3 control samples.

2013-07-04 | E-GEOD-48188 | biostudies-arrayexpress

Analysis of global gene expression in uninduced and β-estradiol treated G1E-ER-GATA cells

Project description:Total RNA was analyzed from either uninduced or β-estradiol treated G1E-ER-GATA cells to determine changes in gene expression upon induction of erythroid maturation (treated).

2009-11-25 | GSE18870 | GEO

SetD8 Knockdown in ß-estradiol-treated (24 h) G1E-ER-GATA-1 cells

Project description:The establishment and maintenance of cell type-specific transcriptional programs require an ensemble of broadly expressed chromatin remodeling and modifying enzymes. Many questions remain unanswered regarding the contributions of these enzymes to specialized genetic networks that control critical processes such as lineage commitment and cellular differentiation. We have been addressing this problem in the context of erythrocyte development driven by the transcription factor GATA-1 and its coregulator Friend of GATA-1 (FOG-1). As certain GATA-1 target genes have little to no FOG-1 requirement for expression, presumably additional coregulators can mediate GATA-1 function. Using a genetic complementation assay and RNA interference in GATA-1-null cells, we demonstrate a vital link between GATA-1 and the histone H4 lysine 20 methyltransferase PR-Set7/SetD8 (SetD8). GATA-1 selectively induced H4 monomethylated lysine 20 at repressed, but not activated, loci, and endogenous SetD8 mediated GATA-1-dependent repression of a cohort of its target genes. GATA-1 utilized different combinations of SetD8, FOG-1, and the FOG-1-interacting Nucleosome Remodeling and Deacetylase (NuRD) complex component Mi2b to repress distinct target genes. Implicating SetD8 as a context-dependent GATA-1 corepressor expands the repertoire of coregulators mediating establishment/maintenance of the erythroid cell genetic network and provides a biological framework for dissecting the cell type-specific functions of this important coregulator. We propose a coregulator matrix model in which distinct combinations of chromatin regulators are required at different GATA-1 target genes, and the unique attributes of the target loci mandate these combinations.

2013-07-24 | GSE49174 | GEO

SetD8 Knockdown in M-CM-^_-estradiol-treated (24 h) G1E-ER-GATA-1 cells

2013-07-24 | E-GEOD-49174 | biostudies-arrayexpress

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