Transcriptomics,Genomics

Dataset Information

43

Genome-wide analysis of bone marrow-derived macrophage (BMDM) priming by Ifng and Ifnb.


ABSTRACT: Macrophages are a heterogeneous population of immune cells, which are critical for both the initiation and resolution of inflammation. Pro-inflammatory macrophages can be induced by the Th1 cytokine IFNγ and/or TLR triggers, like LPS. Here, we investigated the effects of IFNγ priming on LPS-induced gene expression in primary mouse macrophages. Overall design: To investigate the effect of IFNγ priming on LPS-induced gene expression in macrophages, we stimulated bone marrow-derived macrophages with IFNγ or IFNb for 18 hours and subsequently activated them with LPS for 6 hours.

INSTRUMENT(S): Illumina MouseRef-8 v2.0 expression beadchip

SUBMITTER: Brendon Paul Scicluna  

PROVIDER: GSE60290 | GEO | 2015-03-26

SECONDARY ACCESSION(S): PRJNA257967

REPOSITORIES: GEO

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Publications

IFN-γ priming of macrophages represses a part of the inflammatory program and attenuates neutrophil recruitment.

Hoeksema Marten A MA   Scicluna Brendon P BP   Boshuizen Marieke C S MC   van der Velden Saskia S   Neele Annette E AE   Van den Bossche Jan J   Matlung Hanke L HL   van den Berg Timo K TK   Goossens Pieter P   de Winther Menno P J MP  

Journal of immunology (Baltimore, Md. : 1950) 20150306 8


Macrophages form a heterogeneous population of immune cells, which is critical for both the initiation and resolution of inflammation. They can be skewed to a proinflammatory subtype by the Th1 cytokine IFN-γ and further activated with TLR triggers, such as LPS. In this work, we investigated the effects of IFN-γ priming on LPS-induced gene expression in primary mouse macrophages. Surprisingly, we found that IFN-γ priming represses a subset of LPS-induced genes, particularly genes involved in cel  ...[more]

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