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Transcriptome analysis of quorum sensing-dependent genes in Burkholderia gladioli BSR3.

ABSTRACT: In Burkholdeira gladioli BSR3, quorum sensing (QS) signal plays a pivotal role in many bacterial behaviors, including motility, toxin production and oxalogenesis. To understand transcriptional profiling of QS-dependent genes, we carried out RNA-Seq analysis of the wild type B. gladioli BSR3, and a QS-defective mutant under different culture states (10h and 24h after incubation), representing exponential phase and stationary phase.

ORGANISM(S): Burkholderia gladioli

PROVIDER: GSE60490 | GEO | 2017/08/18

SECONDARY ACCESSION(S): PRJNA258401

REPOSITORIES: GEO

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Transcriptome analysis of S. maltophilia in the exponential and stationary phases and after a treatment with quorum sensing autoinducers.

Project description:Stenotrophomonas maltophilia is an emerging opportunistic multidrug-resistant pathogen frequently co-isolated with other relevant nosocomial pathogens in respiratory tract infections. S. maltophilia uses the endogenous DSF quorum sensing (QS) system to regulate virulence processes but can also respond to exogenous AHL signals produced by neighboring bacteria. A whole-transcriptome sequencing analysis was performed for S. maltophilia strain K279a in the exponential and stationary phases and in exponential cultures after a treatment with exogenous DSF or AHLs. Our first analysis reveals that during the beginning of the stationary phase 1673 genes are differentially expressed representing 37% of total genomic content. COG analysis showed that most of these genes were enriched for energetic metabolism processes and regulation of gene expression. In a second analysis, and after adding DSF or AHLs to cultures in exponential phase, 82 and 28 were found deregulated respectively, 22 of which were commonly upregulated by both autoinducers. Interestingly, among these genes affected by both DSFs and AHLs, 14 functional genes were also upregulated in the stationary phase. Gene functions regulated by all tested conditions include lipid and amino acid metabolism, stress response and signal transduction mechanisms, nitrogen and iron metabolism, and adaptation to microoxic conditions. Overall, our findings provide clues on the role that the QS could have in the transition from the exponential to the stationary phase in S. maltophilia and for the bacterial fitness for high-density growth.

2023-05-16 | GSE206442 | GEO

RNA-Seq analysis of quorum sensing-dependent genes in Burkholderia glumae BGR1

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A novel TetR-like transcriptional regulator in Stenotrophomonas maltophilia is involved in fatty acid metabolism, including quorum sensing signal turnover

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RNA-Seq analysis of quorum sensing-dependent genes in Burkholderia glumae BGR1

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Dual Function of Tropodithietic Acid as Antibiotic and Signaling Molecule in Quorum Sensing

Project description:The marine bacterium Phaeobacter inhibens produces tropodithietic acid (TDA), a broad-spectrum antibiotic and anticancer agent. TDA allows P. inhibens to antagonize other bacteria, including several pathogens, and eukaryotes. Since recently antibiotics are also discussed to function as intermicrobial signals. Here we show that ~10% of the genes of P. inhibens are strongly influenced by N-acyl-homoserine lactone (AHL) mediated quorum sensing (QS), switching the bacteriumâs life style from attached to free-living. In an AHL negative mutant of P. inhibens subinhibitory concentrations of TDA caused the same regulatory effect as the AHL. This demonstrates that bacteria can produce antibiotic compounds not only as weapons, but also to substitute their endogenous AHL molecule in QS. The dual function of TDA probably supports the QS system to accelerate regulatory processes and points to a so far neglected role of antibiotics at subinhibitory concentrations in the environment and in microbial interactions. Comparison of whole transcriptomes of wildytype, quorum sensing mutants (pgaI and pgaR) and pgaI grown supplemented with subinhibitory concentration of the antibiotic TDA. RNA isolated in the late exponential growth phase. 4 biological replicates investigated for each strain.

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2016-10-05 | GSE84969 | GEO

Additive Effects of Quorum Sensing Anti-activators on Pseudomonas aeruginosa Virulence Traits and Transcriptome

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