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Genome-wide identification of BRAT- and PUM-associated mRNAs reveals PUM-independent functions for BRAT in translational repression and mRNA degradation during the Drosophila maternal-to-zygotic transition

ABSTRACT: Here, we analyze the RNA-binding preferences of the Drosophila melanogaster BRAT protein using RNAcompete.

ORGANISM(S): synthetic construct

PROVIDER: GSE60498 | GEO | 2015/03/30

SECONDARY ACCESSION(S): PRJNA258416

REPOSITORIES: GEO

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Genome-wide identification of BRAT- and PUM-associated mRNAs reveals PUM-independent functions for BRAT in translational repression and mRNA degradation during the Drosophila maternal-to-zygotic transition

Project description:Here, we analyze the RNA-binding preferences of the Drosophila melanogaster BRAT protein using RNAcompete. In the RNAcompete assay, purified GST-tagged BRAT is incubated with an excess of RNA pool and bound RNA from individual pulldown experiments are directly labeled and hybridized to a custom Agilent 244K microarray.

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The crystal structure of the NHL domain in complex with RNA reveals the molecular basis of Drosophila Brain tumor-mediated gene regulation

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The tumor suppressor Brat controls neuronal lineages by inhibiting the transcription factors Deadpan and Zelda

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Project description: Not available

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