Transcriptomics,Genomics

Dataset Information

29

1.0 mM As(III) stressed wild-type vs (non-stressed) wild-type after 1 hour


ABSTRACT: Arsenic is ubiquitously present in nature and various mechanisms have evolved enabling cells to evade toxicity and acquire tolerance. Herein, we explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and sulfur metabolite profiling. Arsenite exposure affected transcription of genes encoding functions related to protein biosynthesis, arsenic detoxification, oxidative stress defense, redox maintenance and proteolytic activity. Importantly, enzymes involved in sulfate assimilation and glutathione biosynthesis were induced at both gene and protein levels. Kinetic metabolic profiling evidenced a significant increase in the pools of sulfur metabolites as well as elevated glutathione levels. Moreover, the flux in the sulfur assimilation pathway as well as the glutathione synthesis rate strongly increased with a concomitant reduction of sulfur incorporation into proteins. By combining comparative genomics and molecular analyses, we pin-pointed transcription factors that mediate thecore of the transcriptional response to arsenite. Taken together, our data reveals that arsenite-exposed cells channel a large part of assimilated sulfur into glutathione biosynthesis and we provide evidence that the transcriptional regulators Yap1p and Met4p control this response in concert. Keywords: stress Overall design: Stressed wild-type 1mM 1h versus untreated wild-type using direct comparisons.

INSTRUMENT(S): Saccharomyces cerevisiae 6.4K array (Y6.4K), version 4

SUBMITTER: Erik Kristiansson  

PROVIDER: GSE6066 | GEO | 2007-10-18

SECONDARY ACCESSION(S): PRJNA104437

REPOSITORIES: GEO

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Publications

Quantitative transcriptome, proteome, and sulfur metabolite profiling of the Saccharomyces cerevisiae response to arsenite.

Thorsen Michael M   Lagniel Gilles G   Kristiansson Erik E   Junot Christophe C   Nerman Olle O   Labarre Jean J   Tamás Markus J MJ  

Physiological genomics 20070227 1


Arsenic is ubiquitously present in nature, and various mechanisms have evolved enabling cells to evade toxicity and acquire tolerance. Herein, we explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent arsenic (arsenite) by quantitative transcriptome, proteome, and sulfur metabolite profiling. Arsenite exposure affected transcription of genes encoding functions related to protein biosynthesis, arsenic detoxification, oxidative stress defense, redox maintenance, and proteolytic  ...[more]

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