In vivo probing of promoter overlapping between σ-factors in Escherichia coli (ChIP-Seq)

ABSTRACT: An alternative sigma factor (σ32) recognizes the unique set of promoters upon heat shock. Here, we determined 54 σ32 promoters at nucleotide resolution using ChIP-exo, enabling us to compare those with housekeeping σ70 promoters. The results elucidated the overarching principles of promoter overlapping between the two σ-factors, which are sequence-specific non-, half-, and full-shared modes with a perfect sequence conservativeness of −35 element as a key determinant of full-shared mode.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE60668 | GEO | 2020/08/12

REPOSITORIES: GEO