Genomics

Dataset Information

32

LexA mutant vs Shewanella piezotolerans WP3 wild-type strain (20 C)


ABSTRACT: The lexA gene was deleted from WP3 genome to construct the WP3ΔlexA strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Overall design: Three biological replicates sample and 6 technical replicates for each experiment

INSTRUMENT(S): Shewanella piezotolerans WP3 whole genome microarray

SUBMITTER: Huahua Jian  

PROVIDER: GSE60727 | GEO | 2014-12-01

SECONDARY ACCESSION(S): PRJNA259369

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE60727_RAW.tar Raw
GSE60727_jianandy_8211_WP3cy5_lexAcy3.LSR.gz Other
GSE60727_jianandy_9790_WP3cy3_lexAcy5.LSR.gz Other
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Publications

The regulatory function of LexA is temperature-dependent in the deep-sea bacterium Shewanella piezotolerans WP3.

Jian Huahua H   Xiong Lei L   He Ying Y   Xiao Xiang X  

Frontiers in microbiology 20150618


The SOS response addresses DNA lesions and is conserved in the bacterial domain. The response is governed by the DNA binding protein LexA, which has been characterized in model microorganisms such as Escherichia coli. However, our understanding of its roles in deep-sea bacteria is limited. Here, the influence of LexA on the phenotype and gene transcription of Shewanella piezotolerans WP3 (WP3) was investigated by constructing a lexA deletion strain (WP3ΔlexA), which was compared with the wild-ty  ...[more]

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