Dataset Information


LexA mutant vs Shewanella piezotolerans WP3 wild-type strain (20 C)

ABSTRACT: The lexA gene was deleted from WP3 genome to construct the WP3ΔlexA strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Overall design: Three biological replicates sample and 6 technical replicates for each experiment

INSTRUMENT(S): Shewanella piezotolerans WP3 whole genome microarray

SUBMITTER: Huahua Jian  

PROVIDER: GSE60727 | GEO | 2014-12-01



Dataset's files

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GSE60727_RAW.tar Raw
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GSE60727_jianandy_9790_WP3cy3_lexAcy5.LSR.gz Other
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The regulatory function of LexA is temperature-dependent in the deep-sea bacterium Shewanella piezotolerans WP3.

Jian Huahua H   Xiong Lei L   He Ying Y   Xiao Xiang X  

Frontiers in microbiology 20150618

The SOS response addresses DNA lesions and is conserved in the bacterial domain. The response is governed by the DNA binding protein LexA, which has been characterized in model microorganisms such as Escherichia coli. However, our understanding of its roles in deep-sea bacteria is limited. Here, the influence of LexA on the phenotype and gene transcription of Shewanella piezotolerans WP3 (WP3) was investigated by constructing a lexA deletion strain (WP3ΔlexA), which was compared with the wild-ty  ...[more]

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