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Longissimus cervicis muscle of beef steers with divergent Warner Bratzler Shear Force (WBSF) residuals following different post-mortem treatments (electrically stimulated v. non-stimulated)

ABSTRACT: Transcriptional profiling of bovine skeletal muscle comparing expression differences of a population of beef cattle selected for divergent response (shear force residuals) to post-mortem treatment (electrical stimulation)

ORGANISM(S): Bos indicus x Bos taurus Bos taurus

PROVIDER: GSE60935 | GEO | 2015/08/28

SECONDARY ACCESSION(S): PRJNA259861

REPOSITORIES: GEO

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Longissimus cervicis muscle of beef steers with divergent Warner Bratzler Shear Force (WBSF) residuals following different post-mortem treatments (electrically stimulated v. non-stimulated)

Project description:Transcriptional profiling of bovine skeletal muscle comparing expression differences of a population of beef cattle selected for divergent response (shear force residuals) to post-mortem treatment (electrical stimulation) 4 condition experiment utilizing 47 total samples (originally 48, one sample could not be used). Samples were broken in to high/low groups based on residual WBSF following electrical stimulation (ES) and without electrical stimulation (NES). There are 4 groups based on residual WBSF: High-ES (12), Low-ES (11), High-NES (12), and Low-NES (12)

2015-08-28 | E-GEOD-60935 | biostudies-arrayexpress

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Project description:Neotyphodium coenophialum is an endophytic fungus that infects most tall fescue (Festuca arundinacea) pastures that are commonly used in animal grazing systems in the United States. Beef cattle grazing such pastures are impaired in health and production performance, resulting in a large economic loss in US food-animal production systems. Based on the clinical symptoms and laboratory analyses of blood, it was hypothesized that such affected cattle display liver-specific changes in the expression of gene transcripts that are associated with the metabolic enzymes and transporters critical for beef health and performance. Microarray analysis using the GeneChip Bovine Genome Array (Affymetrix, Inc., Santa Clara, CA) was conducted to determine if grazing endophyte-infected tall fescue pastures affects the liver gene expression profiles of growing beef steers. Nineteen steers were assigned to graze either a low toxic endophyte tall fescue-mixed grass (LE treatment, 5.7 ha, n = 9) or a high toxic endophyte infected tall fescue (HE treatment, 5.7 ha, n = 10) pasture located in the University of Kentucky Agricultural Research Center. All steers had ad libitum access to fresh water and an industry standard mineral-vitamin supplement. 88 days grazing on pasture. Approximately 2 g of tissue from the right lobe of the liver of each steer were collected for RNA extraction and microarray analysis.

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