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A dominant-negative isoform of IKAROS expands primitive normal human hematopoietic cells

ABSTRACT: Human cord blood CD34+ cells transduced with control of IK6 lentivirus were transplanted into NSG mice. Control and IK6 transduced CD34+38- progeny were acquired 10 weeks later by FACS

ORGANISM(S): Homo sapiens

PROVIDER: GSE60957 | GEO | 2014/09/14

SECONDARY ACCESSION(S): PRJNA259963

REPOSITORIES: GEO

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A dominant-negative isoform of IKAROS expands primitive normal human hematopoietic cells

Project description:Human cord blood CD34+ cells transduced with control of IK6 lentivirus were transplanted into NSG mice. Control and IK6 transduced CD34+38- progeny were acquired 10 weeks later by FACS Comparison of control and IK6+ human CD34+38- progenitor cells

2014-09-14 | E-GEOD-60957 | biostudies-arrayexpress

A dominant-negative isoform of IKAROS expands primitive normal human hematopoietic cells.

Project description: Not available

| S-EPMC4235152 | biostudies-literature

BCR-ABL1 and a dominant negative isoform of Ikaros cooperate to induce human acute myeloid leukemia

2014-05-23 | GSE45452 | GEO

BCR-ABL1 and a dominant negative isoform of Ikaros cooperate to induce human acute myeloid leukemia

Project description:Analysis of lineage depleted human cord blood cells sequentially transduced with retro- (BCR-ABL1) and lentiviral (Ik6) vectors and the corresponding controls. Results provide important informations on the collaboration of BCR-ABL1 and Ik6 in human hematopoietic cells. Double-transduced lineage depleted human cord blood cells were puirifed according to GFP and LNGFR expression. RNA was extracted from purified cells.

2014-05-23 | E-GEOD-45452 | biostudies-arrayexpress

bulkRNA seq of spleens from humanized mouse comparing NRG to NSG-DKO strains and additionally vaccination using lentiviral constructs

Project description:Decifering why NSG-DKO mice have better humanization upon CD34+ stem cell injection than NRG mice. Additionally, checking effects of lentivirus administration that delivers human HLAs, human cytokines and a viral antigen, which, as a consequence, should improve human adaptive immunity in our huNSG-DKO mouse model.

2024-04-01 | E-MTAB-13883 | biostudies-arrayexpress

BRAFV600E or mutant MAP2K1 human CD34+ cells establish Langerhans cell-like histiocytosis in immunodeficient mice

Project description:We describe a xenograft model for human LCH-like disease in which we transduced human Cord Blood CD34+ HSPCs with a virus coding for BRAF-V600E - the most abundant pathognomonic somatic mutation occuiring in LCH. Cells were subsequently subjected to RNA-sequencing or transplanted into humanized MISTRG or NSG mice.

2020-10-12 | GSE153831 | GEO

Transcriptomic analysis of the in vivo effects of CDK8/19 inactivation in 22Rv1 xenografts growing in intact and castrated NSG mice

Project description:RNA-Seq analysis was carried out to investigate the effects of CDK8/19 inactivation in the tumors formed in intact and castrated NSG mice by different 22Rv1 derivatives, with or without SNX631 treatment. The 22Rv1 derivatives were generated as following: Parental 22Rv1 (Rv1-WT) were transduced with a lentivirus expressing luciferase, yielding the derivative Rv1-Luc. 22Rv1 cells with a double knockout of CDK8 and CDK19 (Rv1-dKO) were made via CRISPR/Cas9. Rv1-dKO cells were further transduced with lentiviruses to generate Rv1-dKO re-expression derivatives that express wild-type CDK19 (Rv1-dKO-CDK19) and the kinase-inactive D173A mutant (Rv1-dKO-CDK19M). SNX631 is a selective and orally bioavalable CDK8/19 inhibtor.

2024-03-31 | GSE240370 | GEO

Genetic analysis of Ikaros target genes and tumor suppressor function in BCR-ABL1+ pre-B ALL [ATAC-Seq]

Project description:Using patient-derived Ph+ pre-B ALL cells that harbor deletions of the IKZF1 DNA binding domain (Ik6), we performed ATAC-sequencing in cells that inducibly express wild-type Ikaros (Ik1) or an empty vector control.

2017-02-06 | GSE90666 | GEO

Genetic analysis of Ikaros target genes and tumor suppressor function in BCR-ABL1+ pre-B ALL [ChIP-seq]

Project description:ChIP-seq was performed in patient-derived BCR-ABL1+ (Ph+) pre-B ALL cells that harbor heterozygote deletions of the IKZF1 DNA binding domain (Ik6), using cells that inducibly express wild-type Ikaros (Ik1) or an empty vector control.

2017-02-06 | GSE90656 | GEO

The gene expression upon dominant negative form of GAS2 (GAS2DN) in MEG-01 cells

Project description:GAS2DN could suppress the growth of chronic myeloid leukemia cells, including K562, MEG-01 and CD34+ cells from patients. In addition, GAS2DN inhibited the tumorigenic ability of MEG-01 cells in nude mice. To understand the molecular insight of this inhibitory effect of GAS2DN, global gene expression were performed. The control and GAS2DN-transduced MEG-01 cells were used for microarray analysis.

2013-07-25 | GSE49184 | GEO

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