Transcriptomics,Genomics

Dataset Information

41

A global transcriptome analysis of keratinocytes upon overexpression of microRNA-132


ABSTRACT: MiR-132 is one of the most upregulated miRNAs in keratinocytes of human skin wounds during the inflammatory phase of healing; however its biological role during skin wound healing has not been studied. To study the genes regulated by miR-132, we transfected miR-132 mimics (pre-miR-132) into primary human keratinocytes to overexpress miR-132. We performed a global transcriptome analysis of keratinocytes upon overexpression of miR-132 using Affymetrix arrays. Overall design: Expression profiling of primary human keratinocytes transfected with 10nM miR-132 mimics (pre-miR-132) or control oligos (pre-miR-Ctrl) for 48 hours (biological triplicates in each group) was performed using Affymetrix GeneTitan system.

INSTRUMENT(S): [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: Ning Xu 

PROVIDER: GSE61139 | GEO | 2014-12-30

SECONDARY ACCESSION(S): PRJNA260313

REPOSITORIES: GEO

Similar Datasets

| GSE87436 | GEO
| GSE92409 | GEO
| GSE93110 | GEO
1000-01-01 | S-EPMC3069485 | BioStudies
2015-01-01 | S-EPMC4539686 | BioStudies
2011-07-01 | E-GEOD-24812 | ArrayExpress
2011-07-01 | GSE24812 | GEO
| GSE46197 | GEO
2012-10-30 | E-GEOD-41905 | ArrayExpress
2019-01-01 | S-EPMC6842347 | BioStudies