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Gene expression and functional annotation of choroid plexus epithelium failure in Alzheimer disease

ABSTRACT: We investigated the gene expression of the human CPE in Braak stadia 0, 3 and 6.We isolated CPE cells with laser dissection microscopy from healthy human donor choroid plexus tissues and from human choroid plexus tissue from brains that were post-mortem staged in Braak 3 and Braak 6 Alzheimer disease stages . Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays.

ORGANISM(S): Homo sapiens

PROVIDER: GSE61196 | GEO | 2014/12/31

SECONDARY ACCESSION(S): PRJNA260739

REPOSITORIES: GEO

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Gene expression of the human choroid plexus epithelium (CPE)

Project description:We investigated the gene expression of the human CPE.We isolated CPE cells from healthy human donor choroid plexus tissues with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 7 replicates of human CPE samples from 7 different donors.

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Cross-species genomics identifies postanatal CPE as novel choroid plexus carcinoma oncogenes.

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