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Drosophila gliogenesis during early embryogenesis - sorted experiment

ABSTRACT: We report here the application of magnetic cell separation in microarray experiment to understand Drosophila gliogenesis during early embryogenesis. At stage 11, neuroectodermal cells were labeled by a fusion transmembrane protein, mCD8-GFP, in the wild-type embryos as wells as in the embryos where glide/gcm was overexpressed. Following the cell dissociation, neuroectodermal cells were purified by using the commercial available anti-mCD8 microbeads. Microarray experiments based on prepared neuroectodermal cells from wild-type embryos verse glide/gcm overexpressed embryos at stage 11 have revealed 76 gene differentially regulated by overexpression of glide/gcm. Among them, there are 11 genes have already been shown to be regulated by glide/gcm in the previous publications. Additionally, we have identified 4 more new glide/gcm-regulated genes by in situ hybridization or immunohistochemical staining. It is very convincing that reduction of the tissue complexity by cell purification leads to low false positives in microarray experiment. Keywords: repeat sample

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE612 | GEO | 2003/10/28

SECONDARY ACCESSION(S): PRJNA85423

REPOSITORIES: GEO

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Project description:Preeclampsia (PE), a major cause of excess maternal and perinatal morbidity and mortality, has a worldwide prevalence of 2-8%. Excess inflammation with immune maladaptation in the maternal decidua causes shallow trophoblast invasion, resulting in incomplete spiral artery transformation that are characteristic of PE. To mimic the separate and combined paracrine effects of IL-11 and IL-6 on trophoblasts in normal versus preeclamptic pregnancies, primary cytotrophoblast (CTB) cultures treated with IL-11 or IL-6 or IL-11+IL-6 were analyzed by whole genome microarray. These microarray analyses revealed that IL-11 treated CTBs displayed a total of 283 differentially expressed genes (159 of which are up-regulated and 124 are down-regulated), whereas IL-6 treated CTBs displayed a total of 243 differentially expressed genes (120 are up-regulated and 123 are down-regulated) compared control group. However, IL-11+IL-6 co-treated CTBs exhibited 276 differentially expressed genes (146 of which are up-regulated and 130 are down-regulated) compared to control group. Since IL-11 and IL-6 can compete to activate target cells through binding their shared receptor, the global transcription repertoire of CTB cultures treated with IL-11+IL-6 was compared to those treated with either IL-11 or IL-6. A total of seven genes are differentially regulated by IL-11+IL-6 co-treatment vs. treatment with IL-11 alone (four of which are up-regulated and three down-regulated), whereas compared to IL-6 alone, a total of six genes are differentially regulated by IL-6+IL-11 (all of which are down-regulated). Microarray results of this study support that co-existence of IL-6 and IL-11 impairs the global transcription repertoire of trophoblasts compared to the presence of IL-11 alone, most probably through a mechanism that partial or complete IL-6 binding to gp130 blocks IL-11 action. Importantly, ISG15 is the only commonly affected gene and is downregulated by IL-6 and IL-11 co-treatment compared to control or treatment with either IL-11 or IL-6 alone, suggesting its involvement in PE.

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Drosophila gliogenesis during early embryogenesis - sorted experiment

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