Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Distribution of H3K27me2, dUTX, and histone H3 in Sg4 cells

ABSTRACT: PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Less well known but quantitatively much more important is the genome-wide role of PRC2 that dimethylates more than half of total H3K27. Here we have determined genomic distributions of H3K27me2, dUTX, and histone H3 in cultured Drosophila Sg4 cells by hybridization of ChIP products with tiling microarrays.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE61261 | GEO | 2015/05/14

SECONDARY ACCESSION(S): PRJNA260610

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Distribution of H3K27me2, dUTX, and histone H3 in Sg4 cells

2015-05-14 | E-GEOD-61261 | biostudies-arrayexpress

Genome-wide analysis of epigenetic marks in E(z) temperature sensitive cells [ChIP-seq]

Project description:PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Less well known but quantitatively much more important is the genome-wide role of PRC2 that dimethylates ~70% of total H3K27. Here we show that H3K27me2 occurs in inverse proportion to transcriptional activity in genes and intergenic regions and its loss results in global transcriptional derepression proportionally greatest in previously silent or weakly transcribed regions. H3K27me2 levels are controlled by opposing roaming activities of PRC2 and the H3K27 demethylase dUTX. Unexpectedly, we find an equally pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub), attributed to the RING1 subunit of PRC1-type complexes. Examination of global changes in five epigenetic marks when E(z) is inactivated in E(z) temperature-sensitive cells at 25°C and 31°C. Genome-wide binding of Polycomb was also examined to classify canonical PcG target regions.

2015-05-14 | E-GEOD-61308 | biostudies-arrayexpress

Genome-wide analysis of histone marks in E(z) temperature sensitive cells [ChIP-seq]

2015-05-14 | E-GEOD-64219 | biostudies-arrayexpress

Genome-wide analysis of whole transcriptome in E(z) temperature sensitive cells [RNA-Seq]

Project description:PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Less well known but quantitatively much more important is the genome-wide role of PRC2 that dimethylates ~70% of total H3K27. Here we show that H3K27me2 occurs in inverse proportion to transcriptional activity in genes and intergenic regions and its loss results in global transcriptional derepression proportionally greatest in previously silent or weakly transcribed regions. H3K27me2 levels are controlled by opposing roaming activities of PRC2 and the H3K27 demethylase dUTX. Unexpectedly, we find an equally pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub), attributed to the RING1 subunit of PRC1-type complexes. Examination of global changes in transcription genome-wide when E(z) is inactivated by monitoring total RNA from E(z) temperature-sensitive cells at 25°C and 31°C in duplicate

2015-05-14 | E-GEOD-61307 | biostudies-arrayexpress

Genome-wide analysis of histone marks in E(z) temperature sensitive cells [ChIP-seq]

2015-05-14 | GSE64219 | GEO

Genome-wide analysis of epigenetic marks in E(z) temperature sensitive cells [ChIP-seq]

2015-05-14 | GSE61308 | GEO

Genome-wide analysis of whole transcriptome in E(z) temperature sensitive cells [RNA-Seq]

2015-05-14 | GSE61307 | GEO

Distribution of H3K27me3, H3K27ac, and H3K4me1 in E(z) temperature sensitive cells

2015-05-14 | GSE61262 | GEO

Distribution of H3K27me3, H3K27ac, and H3K4me1 in E(z) temperature sensitive cells

Project description:PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Here we have determined genomic distributions of H3K27me3, H3K27ac, and H3K4me1 in cultured Drosophila E(z) temperature sensitive cells at 25°C and 31°C where E(z) is largely inactivated by hybridization of ChIP products with tiling microarrays. Genomic distributions of H3K27me3, H3K27ac, and H3K4me1 in cultured Drosophila E(z) temperature sensitive cells (EZ2-2) at 25°C and 31°C where E(z) is largely inactivated by hybridization of ChIP products with tiling microarrays

2015-05-14 | E-GEOD-61262 | biostudies-arrayexpress

Distinct Roles of H3K27me2 and H3K27me3 States Unveiled By Fate Specification of Embryonic Stem Cells

Project description:The Polycomb Repressive Complex 2 (PRC2) induces methylation of lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have indeed different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESC) with a modified H3K27me2/H3K27me3 ratio. Our findings document a developmental dynamic control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESC. They also reveal that modifying the H3K27me2/H327me3 ratio is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes, along with influencing induction of the ESC ground state.

2016-09-01 | GSE85717 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data