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Alteration of differentiation potentials by modulating GATA transcription factors in murine embryonic stem cells

ABSTRACT: Alteration of differentiation potentials by modulating GATA transcription factors in murine embryonic stem cells Keywords: retinoic acid-induced differentiation in GATA4,5,6 (-/-) cell lines

ORGANISM(S): Mus musculus

PROVIDER: GSE6133 | GEO | 2006/10/31

SECONDARY ACCESSION(S): PRJNA97639

REPOSITORIES: GEO

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Alteration of Differentiation Potentials by Modulating GATA Transcription Factors in Murine Embryonic Stem Cells.

Project description: Not available

| S-EPMC2956456 | biostudies-literature

Mus musculus

Project description:Alteration of differentiation potentials by modulating GATA transcription factors in murine embryonic stem cells

| PRJNA97639 | ENA

ChIP seq for GATA-1 and HDAC1 in G1E cells with wild type GATA-1 or mutant

Project description:The transcription factor GATA-1 is essential for erythroid and megakaryocytic cell differentiation and maturation. Previous reports show that GATA-1 is modulated through acetylation modification and through FOG-1 mediated indirect intereaction with HDAC1/2 containing NuRD corepressor complexes. In this study, we found that NuRD does not deacetylate GATA-1. However, HDAC1 alone can efficiently deacetylates GATA-1 and the direct interaction of HDAC1 and GATA-1 is required for the deacetylation. Two arginines within GATA-1 linker region are important for this interaction and arginine to alanine mutations (2RA mutant) largely reduces GATA-1 binding to HDAC1 in FOG-1 independent manner. GATA-1 2RA mutant were then introduced into G1E cells, a GATA-1-null erythroid progenitor cells. The 2RA mutant is acetylated but fails to induce erythroid differentiation. Gene expression analysis shows that GATA-1 2RA mutant affects GATA-1 function in both GATA-1 activated and repressed genes. The gene expression pattern partially overlap with gene expression profile of GATA-1V205M, a GATA-1 mutant with defective FOG-1 binding. ChIP-seq analysis further reveal that 2RA mutation largely reduced GATA-1 chromatin binding, most profoundly at gene promoter regions. HDAC1 recruitment on those promoters are also strongly reduced. These results revealed that GATA-1 recruits HDAC1 to GATA-1 regulated gene promoters and HDAC1 is required for GATA-1 mediated transcription regulation.

2021-09-29 | GSE161608 | GEO

Role of GATA-2 -77 enhancer in murine blood progenitor cells

Project description:We discovered enhancers that control GATA-2 expression during mouse development and in adult and generated mutant mouse strains that lack these enhancers, and these mice have very specific and important phenotypes that revealed GATA-2 mechanisms to control stem and progenitor cell transitions. In this study we aim to determine what proteins are under control of the GATA-2 -77 enhancer in a pool of heterogeneous blood progenitor cells with the specific focus on Socs2 expression.

2020-06-05 | PXD013855 | Pride

BMP signaling cooperates with GATA factors to govern stage-specific gene expression during erythroid differentiation [RNA-Seq]

Project description:Hematopoietic progenitors respond to developmental and environmental cues to differentiate. The stage-specific steps of differentiation are stereotypic for each cell lineage, and are controlled by transcription. Here we investigate how differential genomic binding of signal-responsive and lineage-restricted transcription factors can specify subsequent stages of erythropoiesis. Using a human erythroid differentiation system, we extensively characterized the co-operation of the BMP signaling transcription factor SMAD1 with the erythroid transcription factors GATA2 and GATA1 in a detailed time-course. BMP signaling enhances erythroid differentiation. SMAD1 is co-recruited with GATA factors at stage-specific genes that are required to have high expression in each stage. Additionally, we observed SMAD1-GATA co-enriched regions are within super-enhancers and span accessible chromatin. Co-bound regions harbor cell-type specific transcription factor motifs that change during commitment, in contrast to GATA-alone regions. Our studies demonstrate that signal-responsive factors together with lineage regulators mark genomic “hotspots” that function to regulate stage-specific gene expression.

2020-09-14 | GSE74482 | GEO

BMP signaling cooperates with GATA factors to govern stage-specific gene expression during erythroid differentiation [ChIP-Seq]

2020-09-14 | GSE74479 | GEO

BMP signaling cooperates with GATA factors to govern stage-specific gene expression during erythroid differentiation [ATAC-Seq]

2020-09-14 | GSE74472 | GEO

Genome-wide binding analysis of Arabidopsis GATA transcription factors GNC and CGA1

Project description:GATA transcription factors are involved in multiple processes in plant growth and development. Two GATA factors, nitrate-inducible, carbon metabolism-involved (GNC) and CYTOKININ-RESPONSIVE GATA FACTOR 1 (CGA1, also named GNL) are important regulators in greening, flowering, senescence and hormone signaling. However, their direct target genes in regulating these biological processes are poorly characterized. Here, we report a genome-wide identification of the target genes of Arabidopsis GNC and CGA1.

2017-10-01 | GSE97499 | GEO

The role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation

2012-03-05 | GSE35695 | GEO

The role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation

Project description:We explored the role of FOG-1 in GATA-1 transcriptional regulation of megakaryocyte differentiation through expression of wild-type GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells. G1ME cells were derived from Gata1-null mouse ES cells and have both megakaryocyte and erythrocyte differentiation potential upon reconstitution of GATA-1 expression (Stachura 2006). HA-tagged wild-type or mutant GATA-1 were expressed in G1ME cells grown in TPO via retroviral transductions. The cells were sorted for GFP positivity 68 hours post-transduction and then were allowed to recover in normal growth medium for 4h. Total RNA was then isolated using RNeasy kit from Qiagen 72 hours post-transduction.

2012-03-05 | E-GEOD-35695 | biostudies-arrayexpress

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