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Transcriptome of Arabidopsis thaliana ceh1 mutant

ABSTRACT: Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE61675 | GEO | 2014/09/24

SECONDARY ACCESSION(S): PRJNA261814

REPOSITORIES: GEO

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Transcriptome of Arabidopsis thaliana ceh1 mutant

Project description:Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade. About 20 two-week-old plants grown on 1/2 MS medium were collected to extract total RNA. Transcriptome of parent line and ceh1 mutant were compared.

2014-09-24 | E-GEOD-61675 | biostudies-arrayexpress

Proteome of Arabidopsis thaliana eds16, ceh1, and eds16 ceh1 mutants

Project description:Here we present the proteomic profile of mutant plants designated as eds16 (enhanced disease susceptibility), ceh1 (constitutively expressing HPL) as well as the ceh1 eds16 double mutant. CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade.

| MSV000078868 | MassIVE

CSB3 transcriptome analysis (Arabidopsis thaliana)

Project description:In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens. 5 five-week-old plants were collected, frozen in liquid nitrogen and stored at -80ÂºC. Samples from Col-0 and csb3 were compared.

2014-04-25 | E-GEOD-35507 | biostudies-arrayexpress

CSB3 transcriptome analysis (Arabidopsis thaliana)

2014-04-25 | GSE35507 | GEO

Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions

Project description:To obtain an interpretation from the view of transcriptome on distinct metabolite accumulation between ecologically different regions in China, next-generation sequencing technology was performed on E-L 31, 35，36 and 38 stages of Muscat Blanc a Petits Grains grape berries from Changli (CL, eastern) and Gaotai (GT, western). Transcriptome analysis revealed that some key genes involved in terpene synthesis were markedly up-regulated in the CL region. Particularly in the MEP pathway, the expression of VvHDR (1-hydroxy-2-methyl-2-butenyl 4-diphosphate reductase) paralleled with the accumulation of terpenes, which can promote the flow of isopentenyl diphosphate (IPP) into the terpene synthetic pathway. The glycosidically bound monoterpenes accumulated differentially along with maturation in both regions, which is synchronous with the expression of a monoterpene glucosyltransferase gene (VvGT14). Other genes were also found to be related to the differential accumulation of terpenes and monoterpene glycosides in the grapes between regions. Transcription factors that could regulate terpene synthesis were predicted through gene co-expression network analysis. Additionally, the genes involved in abscisic acid (ABA) and ethylene signal responses were expressed at high levels earlier in GT grapes than in CL grapes.

2015-07-22 | GSE71146 | GEO

Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions

Project description:To obtain an interpretation from the view of transcriptome on distinct metabolite accumulation between ecologically different regions in China, next-generation sequencing technology was performed on E-L 31, 35?36 and 38 stages of Muscat Blanc a Petits Grains grape berries from Changli (CL, eastern) and Gaotai (GT, western). Transcriptome analysis revealed that some key genes involved in terpene synthesis were markedly up-regulated in the CL region. Particularly in the MEP pathway, the expression of VvHDR (1-hydroxy-2-methyl-2-butenyl 4-diphosphate reductase) paralleled with the accumulation of terpenes, which can promote the flow of isopentenyl diphosphate (IPP) into the terpene synthetic pathway. The glycosidically bound monoterpenes accumulated differentially along with maturation in both regions, which is synchronous with the expression of a monoterpene glucosyltransferase gene (VvGT14). Other genes were also found to be related to the differential accumulation of terpenes and monoterpene glycosides in the grapes between regions. Transcription factors that could regulate terpene synthesis were predicted through gene co-expression network analysis. Additionally, the genes involved in abscisic acid (ABA) and ethylene signal responses were expressed at high levels earlier in GT grapes than in CL grapes. cDNA libraries generated from four developmental stages (E-L 31, 35, 36 and 38) of Muscat Blanc a Petits Grains grape berries in two consecutive years from Changli (CL) and Gaotai (GT) in China were sequenced using Illumina HiSeq™ 2000.Only one RNA-seq library was constructed for each of the E-L31 and E-L36 stage samples because of the small amount of high quality RNA acquired, whereas two libraries were constructed for each of the E-L35 and E-L38 stage samples. As a result, a total of 24 libraries were obtained for the two regions within two years.

2015-07-22 | E-GEOD-71146 | biostudies-arrayexpress

Expression response of Brucella melitensis strain 16M (ATCC23456) to erythritol

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FDPS-regulated transcriptome profile revealed its potential functions in osteoporosis as an RNA binding protein

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2023-02-06 | GSE151605 | GEO

Expression response of Brucella melitensis strain 16M (ATCC23456) to erythritol

Project description:The four-carbon sugar erythritol is an important component of B. melitensis pathogenesis. To determine the transcriptional response to erythritol, B. melitensis strain 16M was grown in the presence of either glucose or erythritol as a sole carbon source. Two control samples (glucose) and two experimental samples (erythritol) were analyzed on Nimblegen B. melitensis microarray chips.

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Arabidopsis proteome in the tripartite interaction between cytokinin, light and temperature

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