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MicroRNA sequencing of HEK293 and H9c2 cells

ABSTRACT: The human HEK293 / 293T and rat cardiomyoblast H9c2 cell lines are commonly employed for microRNA-mRNA interaction studies. Here, I provide microRNA sequencing data obtained from each of these lines to better document which microRNAs are endogenously expressed at high or low levels.

ORGANISM(S): Rattus norvegicus Homo sapiens

PROVIDER: GSE61770 | GEO | 2014/09/26

SECONDARY ACCESSION(S): PRJNA262085

REPOSITORIES: GEO

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microRNA sequencing of HEK293 and H9c2 cells

Project description:The human HEK293 / 293T and rat cardiomyoblast H9c2 cell lines are commonly employed for microRNA-mRNA interaction studies. Here, I provide microRNA sequencing data obtained from each of these lines to better document which microRNAs are endogenously expressed at high or low levels. Small RNA sequencing profiles were generated from cultured HEK293 and H9c2 cells on Illumina HiSeq 2000 instruments.

2014-09-26 | E-GEOD-61770 | biostudies-arrayexpress

microRNA sequencing of HEK293 and H9c2 cells

Project description:microRNA sequencing of HEK293 and H9c2 cells

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H9c2 cell response after retinoic acid stimuli

Project description:During culture, H9c2 cells acquire a myotubule phenotype where a critical component is the inclusion of retinoic acid (RA). The results from some authors on H9c2 suggested that thousands of genes respond to RA stimuli, while other authors report hundreds of genes responding to RA over different cell types. We investigated the response to RA in H9c2 cells controlling for culture time.

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Genome-wide maps in H9C2 cells with or without PAGln treatment (100 μM)

Project description:Analysis of H9C2 cells following PAGln treatment or not. PAGln regulates various mRNA expression in H9C2 cells.Results provide insight into the role of PAGln-involved mechanisms underlying PAGln-mediated effects on coronary artery disease (CAD).

2024-03-31 | GSE228046 | GEO

Investigation of differential microRNAs in the exosomes of alcohol-induced H9c2 myocardial cells

Project description:We use the illumina high-throughput sequencing technology to identify miRNAs between the exosomes of H9c2 cells with or without alcohol-induced.The H9c2 cells were cultured in serum-free medium and stimulated with ethanol (100 mmol/L) or PBS for 24 h, then collected the exosomes samples from serum-free medium. Exosomes were isolated and extracted by differential centrifugation and detected by electron microscopy, particle size and related marker proteins.In total, 123 differentially expressed miRNAs (12 upregulated and 111 downregulated) were screened by miRNA sequence.

2022-01-15 | GSE193545 | GEO

HEK293 stably expressing Autoimmune regulator protein

Project description:Auto Immune REgulator (AIRE) protein expression in HEK293 cells Three cell-lines where used in the study: HEK293-NC; control cell-line expressing yellow fluorescent protein (YFP) HEK-AIRE1, HEK-AIRE2; two different clone-derived Autoimmune regulator protein expressing cell-lines HEK-AIRE-D312A; cell line expressing Autoimmune regulator protein with D312A mutation in the first PHD finger With all cell-lines three independent experiments were performed.

2010-05-05 | E-GEOD-16877 | biostudies-arrayexpress

Effect of the exosomes from cardiopulmonary progenitors on gene expression of H9C2 cells

Project description:To investigate the function of exosomes from cardiopulmonary progenitors in the regulation of gene expression in endothelial cells.We performed gene expression profiling analysis using data obtained from RNA-seq of H9C2 treated with or without CPPs exosomes.

2024-05-08 | GSE232989 | GEO

miRNA-seq of HEK293 cell lines on Illumina iSeq100 instrument

Project description:A validation experiment performed on HEK293 cell lines after genome editing. The design contains three duplicate runs consisted of: HEK293 wild type cell line HEK293 with MIR484 gene knockdown using CRISPR-Cas9 HEK293 with MIR185 gene knockout using CRISPR-Cas9

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IP of endogenous SUMO2/3 in HEK293 cells

Project description:Aim of the experiments was to identify endogenously SUMO2/3 modified proteins in HEK293 cells. Cells were treated with dexamethasone (dex) or vehicle (etoh) and with heatshock (+43C, HS) or normal growth temperature (+37C, non-HS). Cells were induced with tetracycline to express BirA*-GR (glucocorticoid receptor) to express GR at sufficient levels to assess effect of GR activation on protein SUMOylation.

2021-01-11 | PXD017714 | Pride

Transcriptional profiling of apolipoprotein-O-overexpressing H9c2 cardiomyoblasts

Project description:Mitochondrial dysfunction and excessive lipid accumulation in non-adipose tissues have been proposed widely as the roots for comorbidities generated by the growing epidemia of type 2 diabetes mellitus. Mouse models of lipotoxic cardiomyopathy have underlined this detrimental situation, but so far the proteins involved in diabetic patients’s induced mitochondrial dysfunction remain unknown. Apolipoprotein O (ApoO), originally found overexpressed in human diabetics hearts (Lamant et al. JBC 2006), is a candidate that was investigated here at the transcriptome level using H9c2 cardiomyoblasts after stable integration of an expression vector (pTT-ApoO) constitutively expressing ApoO.

2014-12-30 | GSE34076 | GEO

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