Stress response of Saccharomyces cerevisiae to exposure to natural product pyocyanin.
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
PROVIDER: GSE6185 | GEO | 2007/01/22
SECONDARY ACCESSION(S): PRJNA100657
REPOSITORIES: GEO
Similar Datasets
Project description:Pyocyanin has been shown to engage in redox transfer of electrons from NADPH to oxygen to generate superoxide radicals. Transcriptional response to oxygen stress has been characterized in yeast and should be observable upon exposure to pyocyanin if this is the true mode of action. We used microarrays to detail the global programme of gene expression induced in the stress response to pyocyanin. Experiment Overall Design: Saccharomyces cerevisiae cells were exposed to 25 and 250 ug/ml of pyocyanin for a period of 6 hours incubation for RNA extraction and hybridization on Affymetrix microarrays. Results were compared to transcription levels in untreated cells under the same incubations conditions. The data indicated that transcriptional changes were consistent with an oxidative stress response, as observed by other researchers investigating the mode of action of pyocyanin toxicity.
2008-06-14 | E-GEOD-6185 | biostudies-arrayexpress
Project description:The mechanism by which plants regulate channeling of photosynthetically derived electrons into different areas of chloroplast metabolism remains obscure. In higher plants, these electrons are made accessible to stromal enzymes, or cyclic electron flow, as reduced ferredoxin (Fd), or NADPH. We investigated how the specific loss of an Arabidopsis (Arabidopsis thaliana) ferredoxin:NADPH reductase (FNR) isoprotein affected channeling of photosynthetic electrons into NADPH and Fd dependent metabolism. Affymetrix gene chip comparison of transcript changes in these mutants against two different ecotype backgrounds indicates that stress signaling is perturbed, and experiments revealed that the mutants are more susceptible to oxidative stress. Keywords: mutant wild type transcript profile comparison
2008-10-11 | GSE9402 | GEO
Project description:The mechanism by which plants regulate channeling of photosynthetically derived electrons into different areas of chloroplast metabolism remains obscure. In higher plants, these electrons are made accessible to stromal enzymes, or cyclic electron flow, as reduced ferredoxin (Fd), or NADPH. We investigated how the specific loss of an Arabidopsis (Arabidopsis thaliana) ferredoxin:NADPH reductase (FNR) isoprotein affected channeling of photosynthetic electrons into NADPH and Fd dependent metabolism. Affymetrix gene chip comparison of transcript changes in these mutants against two different ecotype backgrounds indicates that stress signaling is perturbed, and experiments revealed that the mutants are more susceptible to oxidative stress. Experiment Overall Design: We isolated arabidopsis mutants of the FNR1 gene in different ecotypes, extracted RNA from these and respective wild type plants and hybridised it to Affymetrix microarrays
2008-10-26 | E-GEOD-9402 | biostudies-arrayexpress
Project description:Investigating the oxidative stress response: Candida glabrata strains were stressed with hydrogen peroxide and menadione (causing oxygen radicals) to induce the oxidative stress regulon, which is thought to be upregulated during the oxidative burst inside of phagocytic cells.
2011-01-01 | E-MEXP-2915 | biostudies-arrayexpress
Project description:Global Gene Expression Analysis Reveals Differences in Cellular Responses to Hydroxyl- and Superoxide-induced Oxidative Stress in Caco-2 Cells. Reactive oxygen species-induced oxidative stress in the colon is involved in inflammatory bowel diseases and is suggested to be associated with colorectal cancer risk. However, our insight in molecular responses to different oxygen radicals is still fragmentary. Therefore, we studied global gene expression by an extensive time serie (0.08, 0.25, 0.5, 1, 2, 4, 8, 16, or 24 hours ) analyses in human colon cancer (Caco-2) cells after exposure to H2O2 or menadione, leading to the formation of HO. or O2.- radicals respectively. Next to gene expression, induction of pathways and correlations with related phenotypic markers (oxidative DNA damage, cell cycle arrest) was investigated. Gene expression analysis resulted in 1404 differentially expressed genes upon H2O2 challenge and 979 genes after menadione treatment. Time-dependent co-regulated genes immediately showed a pulse-like response to HO. formation while the O2.--induced expression is not restored over 24 hours. Pathway analyses demonstrated that the difference in the modulation of gene expression is also reflected in regulation of pathways by HO. and O2.-: H2O2 immediately influences pathways involved in the immune function, while menadione constantly regulated cell cycle-related pathways. Altogether, this study offers a novel and detailed insight in differential time-dependent oxidative stress response, but most importantly, shows that effects of HO. and O2.- can also be discriminated regarding their modulation of particular carcinogenesis-related mechanisms. Keywords: Comparison of genome-wide gene expression between different time points for H2O2 and menadione.
2010-01-12 | GSE15327 | GEO
Project description:Small nucleolar RNAs (snoRNAs) guide nucleotide modifications of cellular RNAs in the nucleus. We have previously shown that box C/D snoRNAs from the Rpl13a locus are unexpected mediators of physiologic oxidative stress, independent of their predicted ribosomal RNA modifications. Here, we demonstrate that oxidative stress induced by doxorubicin causes rapid cytoplasmic accumulation of the Rpl13a snoRNAs through a mechanism that requires superoxide and a nuclear splice variant of NADPH oxidase. RNA-sequencing analysis reveals that box C/D snoRNAs as a class are present in the cytoplasm, where their levels are dynamically regulated by NADPH oxidase. These findings suggest that snoRNAs may orchestrate the response to environmental stress through molecular interactions outside of the nucleus.
2015-03-20 | GSE67050 | GEO
Project description:NADPH oxidases are the only know enzyme family that has reactive oxygen species (ROS, e.g. superoxide and hydrogen peroxide) as their main metabolic product. They are therefore a prima candidate gene family to define a molecular source for ROS in physiology and pathophysiology. The type 4 NADPH oxidase (NOX4) is the most abundant isoform with the highest basal expression levels.The Nox4 knockout mouse was designed using a cre/lox recombination technique which allows to create either constitutive (type III recombination) and conditional (type I recombination) knockout alleles.
2011-01-01 | GSE20400 | GEO
Project description:NADPH oxidases are the only know enzyme family that has reactive oxygen species (ROS, e.g. superoxide and hydrogen peroxide) as their main metabolic product. They are therefore a prima candidate gene family to define a molecular source for ROS in physiology and pathophysiology. The type 4 NADPH oxidase (NOX4) is the most abundant isoform with the highest basal expression levels.The Nox4 knockout mouse was designed using a cre/lox recombination technique which allows to create either constitutive (type III recombination) and conditional (type I recombination) knockout alleles. cDNA microarray experiments of brain, kidney, muscle and pancreas of four male mutant mice versus a RNA pool of four male wild type mice. For each animal two technical replicates were performed including a dye swap experiment. All animals with the same age of 17 weeks on a C57BL/6 background.
2011-01-01 | E-GEOD-20400 | biostudies-arrayexpress
Project description:Wild type systemic leaf wound response vs. response in mutants lacking NADPH oxidase (Rboh) Keywords = reactive oxygen species Keywords: other
2004-02-17 | GSE917 | GEO
Project description:We identified a small zinc finger protein, MBS, as a new mediator of singlet oxygen responses in Chlamydomonas and Arabidopsis. MBS is required for induction of singlet oxygen-dependent gene expression and, upon oxidative stress, accumulates in distinct granules in the cytosol of Arabidopsis cells. First, we recorded changes in light stress-regulated gene expression profiles after genetically perturbing MBS function by isolating mutants for the two MBS genes (MBS1 and MBS2) and by overexpression of MBS1 in Arabidopsis thaliana. Then, these light stress-related gene expression profiles were analyzed with respect to genes specifically responding to singlet oxygen and hydrogen peroxide/superoxide. The results indicated that MBS inactivation leads to an impaired response to singlet oxygen signaling under light stress.
2014-04-21 | GSE49596 | GEO