Transcriptomics,Genomics

Dataset Information

24

Gene Transcription Profile of Local Innate and Adaptive Immunity during Early Phase of IBV Infection


ABSTRACT: To understand the mechanistic basis of local innate and adaptive immunity against infectious bronchitis virus (IBV) at the molecular level, we examined the gene transcription profile of tracheal epithelial layers at 3 days after infection of chickens with an attenuated IBV-Massachusetts strain. Keywords: Disease State Analysis, Early mucosal immune response, FHCRC 13k chicken array Overall design: For the DNA microarray experiment, total RNA was reverse transcribed and labeled with Cy3 and Cy5 fluorescent dyes and hybridized to the chicken 13K cDNA microarray (FHCRC, Seattle, WA) which contains 13,000 genes derived from both embryonic and adult chicken tissues including immune genes. The experiment was repeated four times using swapped Cy3 or Cy5 dye labeling.

INSTRUMENT(S): FHCRC Chicken 13K v2.0

SUBMITTER: Xiuqing Wang  

PROVIDER: GSE6211 | GEO | 2006-11-03

SECONDARY ACCESSION(S): PRJNA100579

REPOSITORIES: GEO

altmetric image

Publications

Transcriptome of local innate and adaptive immunity during early phase of infectious bronchitis viral infection.

Wang Xiuqing X   Rosa Artur J M AJ   Oliverira Henrique N HN   Rosa Guilherme J M GJ   Guo Xueshui X   Travnicek Mark M   Girshick Theodore T  

Viral immunology 20060101 4


To understand the mechanistic basis of local innate and adaptive immunity against infectious bronchitis virus (IBV) at the molecular level, we examined the gene transcription profile of tracheal epithelial layers 3 d after infection of chickens with an attenuated IBV-Massachusetts strain. Results suggested that the transcription levels of 365 genes were either upregulated or downregulated (2-fold and higher) after IBV infection. Among the upregulated 250 genes, 25 were directly immune-related ge  ...[more]

Similar Datasets

2006-11-03 | GSE6198 | GEO
2019-06-18 | E-MTAB-7849 | ArrayExpress
2015-07-31 | E-TABM-1128 | ArrayExpress
2016-02-15 | PXD002936 | Pride
2007-12-25 | GSE9045 | GEO
2007-01-01 | GSE6268 | GEO
2007-01-01 | GSE6270 | GEO
| GSE100802 | GEO
2009-11-04 | E-GEOD-13317 | ArrayExpress
2009-11-05 | GSE13317 | GEO