Radiation effects on a 3D cell culture of primary human keratinocytes and the protective effect of N-acetyl-cysteine (NAC) and the Traditional Chinese Medicine Qingre-Liyan decoction (QYD)

ABSTRACT: We studied the radiation effect on cell culture model of oral mucositis. The musositis model (tissues) used is an organotypic model which consisted of 3-dimensional (3-D) cell cultures of primary human oral keratinocytes. The tissues were purchased from MatTek corporation (Ashland, MA) and were grown on top of microporous membrane and supplemented with MatTek serum free media. These tissues were irradiated with 12 Gy. Six hours after the irradiation the tissues were cut in half. Half of the tissue was used to extract total RNA (RNeasy Plus Mini kit, Qiagen, Germantown, MD) and the other half was used to evaluate the histology and the apoptosis. We had the following groups: Non-Irradiated Tissue, Irradiated Tissue with 12 Gy; Irradiated Tissue with 12 Gy pretreated with N-acetylcysteine (NAC); Irradiated Tissue with 12 Gy pretreated with the Traditional Chinese Medicine Qingre-Liyan decoction (QYD); and Irradiated Tissue with 12 Gy pretreated with 50:50 w/w NAC-QYD.

ORGANISM(S): Homo sapiens

PROVIDER: GSE62397 | GEO | 2014/10/16

SECONDARY ACCESSION(S): PRJNA264014

REPOSITORIES: GEO