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The effects of Serum Amyloid A on gene expression profile in HUVECs

ABSTRACT: This study attemps to study the global effects of Serum Amyloid A (SAA) on human endothelial gene expression profile in different time points. Keywords: time course

ORGANISM(S): Homo sapiens

PROVIDER: GSE6241 | GEO | 2006/11/09

SECONDARY ACCESSION(S): PRJNA97511

REPOSITORIES: GEO

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Transcription profiling of human umbilical vein endothelial cells to investigate the effects of Serum Amyloid A, time series

Project description:This study attemps to study the global effects of Serum Amyloid A (SAA) on human endothelial gene expression profile in different time points. Experiment Overall Design: HUVECs were cultured to confluence in 6-well plates and stimulated with 20 Î¼g/ml of SAA (PeproTech) for 0, 4, 24 and 48 h. To determine the cellular transcript profile in Control (0h) and 3 different SAA groups (S4h, S24h and S48h), the relative abundance of mRNAs in SAA groups were compared with Control by use of Human Genome U133 Plus 2.0 Array (HG-U133 Plus 2.0, Affymetrix)). Each group had 2 individual samples. . In order to obtain the 3 group comparison datasets (S4h/C, S24h/C and S48h/C), expression patterns for each individual SAA sample (4h, 24h and 48h) were compared to each individual control sample. Hence, each group comparison has 4 individual sample comparisons. The robust increased or decreased cut-off was use the Affymetrix recommending "1" or "-1" of the signal log ratio. . If the gene is robust âincreasedâ or âdecreasedâ in 4 individual sample comparisons of the total 4 comparisons, it was selected as the final robust changed in group comparison. Each gene we were interested, real time PCR was carried out to validate the microarray results.

2009-04-30 | E-GEOD-6241 | biostudies-arrayexpress

Structural basis for lipid binding and function by an evolutionally conserved protein, Serum Amyloid A

Project description:Serum amyloid A (SAA) is a plasma protein that transports lipids during inflammation. To explore SAA solution conformations and lipid binding mechanism, we used hydrogen-deuterium exchange mass spectrometry, lipoprotein reconstitution, sequence analysis and molecular dynamics simulations. Solution conformations of lipid-bound and lipid-free mSAA1 at pH~7 agreed in details with the crystal structures but also showed important differences. The results revealed that amphipathic α-helices h1 and h3 comprise a lipid-binding site that is partially pre-formed in solution, is stabilized on lipoproteins, and shows lipid-induced folding of h3. This site sequesters apolar ligands via a concave hydrophobic surface in SAA oligomers. The largely disordered C-terminal region is conjectured to mediate promiscuous binding of other ligands. The h1-h2 linker region forms an unexpected β-hairpin that may represent an early amyloidogenic intermediate. The results establish structural underpinnings for understanding SAA interactions with lipids and other ligands, its evolutional conservation, and its transition to amyloid.

2020-02-17 | PXD016391 | Pride

Antifungal Activity of Mammalian Serum amyloid A1 against Candida albicans

Project description:Human serum amyloid A (SAA) is a major acute phase protein and shows a massive increase of concentration in plasma during inflammation. In the current study, we report that recombinant human and mouse SAA1 (rhSAA1 and rmSAA1) have a potent antifungal activity against the major fungal pathogen Candida albicans. rhSAA1 binds to the cell surface of C. albicans and promotes cell aggregation. At high concentrations, rhSAA1 disrupts the membrane integrity and induces rapid cell death of C. albicans. Further investigation demonstrates that rhSAA1 targets on the cell wall adhesin Als3 of C. albicans. Inactivation of ALS3 in C. albicans leads to remarkably decreased cell aggregation and death upon rhSAA1 treatment, implying that Als3 plays a critical role in SAA1 sensing. Moreover, deletion of the ALS3 transcriptional regulators such as AHR1, BCR1, and EFG1 in C. albicans results in a similar effect on cell responses to that of the als3/als3 mutant upon rhSAA1 treatment. Global gene expression profiling analysis indicates that rhSAA1 has a remarkable impact on the expression of cell wall- and metabolism-related genes in C. albicans. Our finding of the antifungal activity of rhSAA1 against C. albicans expands the function of this protein and would provide new insights into the understanding of the host-Candida interaction during infections.

2019-09-01 | GSE128598 | GEO

Response of quiescent human fibroblasts to different growth factors and serum

Project description:Quiescent human fibroblasts (2091 and Wi-38) were stimulated with different growth factors and serum. Cells were collected at 6 different time points followed by global transcriptional profiling. Keywords: time course, growth factor response, cell line comparison

2006-04-12 | GSE3901 | GEO

12hr, 1d, 3d, and 5d exposure of beta-amyloid to astrocytes

Project description:Cortical type-I astrocytes were cultured on the beta-amyloid peptide 25-35 fragment for 12hr, 1d, 3d, and 5d. Keywords = amyloid astrocytes rat Keywords: time-course

2003-12-09 | GSE856 | GEO

Antifungal Activity of Mammalian Serum amyloid A1 against Candida albicans

Project description:Antifungal Activity of Mammalian Serum amyloid A1 against Candida albicans

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Fibroblast Serum Stimulation Time Course

Project description:Foreskin fibroblasts CRL 2091 (ATCC) were serum starved for 48 hours, and harvested at the indicated time points after switching to media with 10% FBS essentially as described (Iyer et al., 1999). RNA from all of the sampled time points were pooled as reference RNA to compare with RNA from individual time points as described (Iyer et al., 1999). Groups of assays that are related as part of a time series. Keywords: time_series_design

2005-12-31 | GSE3945 | GEO

Effect of serum amyloid A1 (SAA1) on gene expression in primary murine macrophages

Project description:Effect of serum amyloid A1 (SAA1) on gene expression in primary murine macrophages

| PRJNA649232 | ENA

Effect of serum amyloid A1 (SAA1) on gene expression in primary murine macrophages

Project description:Macrophages are important regulators of the immune system and are critically involved in the pathophysiology of many diseases. Serum amlyoidosis is a protein misfolding disease which can follow chronic inflammatory conditions. Deposition of fibrils formed from serum amyloid A1 (SAA1) protein occurs systemically and can lead to severe or even fatal outcomes. Macrophages play a critical role in the misfolding and deposition of SAA1-derived fibrils and the role of macrophages in this context has been studied extensively. It is known that SAA1 can bind to some surface receptors on macrophages and it is taken up by the macrophages and metabolized in the lysosomes. On the other hand, the effect of SAA1 on macrophage physiology has been much less investigated so far. Therefore, we aim to investigate the effect of SAA1 on macrophage gene expression by gene microarray analysis. To this end, primary peritoneal macrophages from NMRI mice were treated with endotoxin-free recombinantly expressed full-length mSAA1 for 6 and 24 h and gene expression was analyzed.

2021-07-20 | GSE155278 | GEO