Transcriptomics,Genomics

Dataset Information

21

Erythroid progenitors stimulated with EPO


ABSTRACT: CD34+ progenitors were isolated from the bone marrow of three healthy volunteers. CD34+CD71+CD45RA- were FACS sorted to enrich for erythroid progenitors. The cells were cultured for four hours with or without EPO in combination with LY294002, and harvested for RNA extraction, amplification and expression analysis. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: EPO Keywords: compound_treatment_design Overall design: Computed

INSTRUMENT(S): SHDZ

SUBMITTER: Stanford Microarray Database   

PROVIDER: GSE6260 | GEO | 2006-11-10

SECONDARY ACCESSION(S): PRJNA100545

REPOSITORIES: GEO

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Publications

PI3K/Akt-dependent Epo-induced signalling and target genes in human early erythroid progenitor cells.

Sivertsen Einar Andreas EA   Hystad Marit Elise ME   Gutzkow Kristine Bjerve KB   Døsen Guri G   Smeland Erlend Bremertun EB   Blomhoff Heidi Kiil HK   Myklebust June Helen JH  

British journal of haematology 20061001 1


Erythropoietin (Epo) is the major regulator of differentiation, proliferation and survival of erythroid progenitors, but the Epo-induced changes in gene expression that lead to these effects are not fully understood. The aim of this study was to examine how Epo, via activation of phosphatidylinositol 3-kinase (PI3K)/Akt, exerts its role in the development of erythroid progenitors from CD34+ cells, and to identify early Epo target genes in human erythroid progenitors. In CD34+ progenitor cells, E  ...[more]

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