Transcriptomics,Genomics

Dataset Information

70

Genome-wide omparism of Terc deficient ntESCs


ABSTRACT: Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis. Generation of pluripotent stem cells from telomerase haplo-insufficient donor cells would provide unique opportunities towards the realization of patient-specific stem cell therapies. Recently, pluripotent human embryonic stem cells (ntESCs) have been efficiently achieved by somatic cell nuclear transfer (SCNT). We tested the hypothesis that SCNT could effectively elongate shortening telomeres of telomerase haplo-insufficient cells in the ntESCs using relevant mouse models. Indeed, telomeres of telomerase haplo-insufficient (Terc+/-) mouse cells are elongated in ntESCs. Moreover, ntESCs derived from Terc+/- cells exhibit naïve pluripotency as evidenced by generation of Terc+/-ntESC clone pups by tetraploid embryo complementation (TEC), the most stringent test of naïve pluripotency. These data suggest that SCNT could offer a powerful tool to reprogram telomeres and to discover the factors for robust restoration of telomeres and pluripotency of telomerase haplo-insufficient somatic cells. Overall design: RNAs from Terc+/+, +/- and -/- contain 3 biological repeatsin each group.

INSTRUMENT(S): Illumina Mouse-8 Expression BeadChip

SUBMITTER: Li-Ying Sung  

PROVIDER: GSE62692 | GEO | 2014-11-30

SECONDARY ACCESSION(S): PRJNA264831

REPOSITORIES: GEO

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Publications

Telomere elongation and naive pluripotent stem cells achieved from telomerase haplo-insufficient cells by somatic cell nuclear transfer.

Sung Li-Ying LY   Chang Wei-Fang WF   Zhang Qian Q   Liu Chia-Chia CC   Liou Jun-Yang JY   Chang Chia-Chun CC   Ou-Yang Huan H   Guo Renpeng R   Fu Haifeng H   Cheng Winston T K WTK   Ding Shih-Torng ST   Chen Chuan-Mu CM   Okuka Maja M   Keefe David L DL   Chen Y Eugene YE   Liu Lin L   Xu Jie J  

Cell reports 20141120 5


Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis. Generation of pluripotent stem cells from telomerase haplo-insufficient donor cells would provide unique opportunities toward the realization of patient-specific stem cell therapies. Recently, pluripotent human embryonic stem cells (ntESCs) have been efficiently achieved by somatic cell nuclear transfer (SCNT). We tested the hypothesis that SCNT could e  ...[more]

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