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Relative spatial heterogeneity revealed by transcriptional profiling of multi-region high-grade glioma samples

ABSTRACT: Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array profiling of 20 primary, multi-region high-grade glioma samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE62802 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA265185

REPOSITORIES: GEO

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Relative spatial heterogeneity revealed by transcriptional profiling of multi-region high-grade glioma samples

Project description:Affymetrix GeneChipÂ® Human Genome U133 Plus 2.0 Array profiling of 20 primary, multi-region high-grade glioma samples. Total RNA was extracted from primary high-grade glioma samples and hybridized to GeneChipÂ® Human Genome U133 Plus 2.0 Array according to the manufacturer's instructions.

2015-12-31 | E-GEOD-62802 | biostudies-arrayexpress

Spatial architecture of high-grade glioma reveals tumor heterogeneity within distinct domains

Project description:High-grade gliomas are aggressive primary brain cancers with poor response to standard regimens, driven by immense heterogeneity. In isocitrate dehydrogenase (IDH) wild-type high-grade glioma (glioblastoma, GBM), increased intra-tumoral heterogeneity is associated with more aggressive disease. Recently, spatial technologies have emerged to dissect this complex heterogeneity within the tumor ecosystem by preserving cellular organization in situ. Here, we construct a high- resolution molecular landscape of GBM and IDH-mutant high-grade glioma patient samples to investigate the cellular subtypes and spatial communities that compose high-grade glioma using digital spatial profiling and spatial molecular imaging. This uncovered striking diversity of the tumor and immune microenvironment, that is embodied by the heterogeneity of the inferred copy- number alterations in the tumor. Reconstructing the tumor architecture revealed brain-intrinsic niches, composed of tumor cells reflecting brain cell types and microglia; and brain-extrinsic niches, populated by mesenchymal tumor cells and monocytes. We further reveal that cellular communication in these niches is underpinned by specific ligand-receptor pairs. This primary study reveals high levels of intra-tumoral heterogeneity in high-grade gliomas, associated with a diverse immune landscape within spatially localized regions.

2023-12-13 | GSE232469 | GEO

Comparison of the transcriptomic profiles of two human glioma cell lines, U87 and Glio6.

Project description:Using Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array, we compared the gene expression profiles between the glioma cell lines U87 and Glio6, cultured at 3% of oxygene.

2018-07-01 | GSE58646 | GEO

Relative spatial homogeneity revealed by transcriptional profiling of multi-region medulloblastoma samples

Project description:Affymetrix Human Gene 1.1 ST Array profiling of 52 primary, multi-region medulloblastoma samples. Total RNA was extracted from primary medulloblastoma samples and hybridized to Affymetrix Human Gene 1.1 ST Arrays (24-Array Plates) according to the manufacturer's instructions.

2015-12-31 | E-GEOD-62803 | biostudies-arrayexpress

Spatial immune heterogeneity of hypoxia-induced exhausted features in high-grade glioma.

Project description: Not available

| S-EPMC8757477 | biostudies-literature

Relative spatial homogeneity revealed by transcriptional profiling of multi-region medulloblastoma samples

Project description:Affymetrix Human Gene 1.1 ST Array profiling of 52 primary, multi-region medulloblastoma samples.

2015-12-31 | GSE62803 | GEO

Spatial genomic heterogeneity in multiple myeloma revealed by multi-region sequencing.

Project description: Not available

| S-EPMC5559527 | biostudies-literature

Effect of disulfiram treatment on pediatric high grade glioma

Project description:RNA-seq analysis was performed to compare gene expression between glioma cells treated with disulfiram and untreated glioma cells.

2019-10-16 | GSE138863 | GEO

CircGLIS3 promotes high-grade glioma invasion via modulating Ezrin phosphorylation

Project description:High-grade glioma is highly aggressive and malignant, resistant to combined therapies and easy to relapse. A better understanding of circRNA biological function in high-grade glioma might contribute to the therapeutic efficacy. Here, a circRNA merely up-regulated in high-grade glioma, circGLIS3 (hsa_circ_0002874, originating from exon 2 of GLIS3), was validated by microarray and qRT-PCR. Functional experiments uncovered that up-regulation of circGLIS3 promoted glioma cell migration and invasion, and showed aggressive characteristics in tumor-bearing mice. Fluorescence in situ hybridization, RNA pull-down, RNA immunoprecipitation and immunohistochemical staining showed that circGLIS3 could promoted Ezrin T567 phosphorylation. Further investigation showed that circGLIS3 could be excreted by glioma through exosomes and induced endothelial cells angiogenesis. This study indicates that circGLIS3 is up-regulated in high-grade glioma and contributes to the invasion and angiogenesis of glioma via promoting Ezrin T567 phosphorylation.

2021-09-22 | GSE165859 | GEO

CircGLIS3 promotes high-grade glioma invasion via modulating Ezrin phosphorylation (microarray)

2021-09-22 | GSE165926 | GEO

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