Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling of whole-blood in Mycobacterium avium subsp. paratuberculosis-infected cattle

ABSTRACT: To provide novel insights into understanding the host-immune response during the different stages of progression of the disease, we performed gene expression profiling in Mycobacterium avium subsp. paratuberculosis infection in cattle.

ORGANISM(S): Bos taurus

PROVIDER: GSE62835 | GEO | 2014/10/31

SECONDARY ACCESSION(S): PRJNA265814

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Global host transcriptional profiles following Mycobacterium avium subsp. paratuberculosis infection in mice

2014-10-31 | GSE62836 | GEO

Global host transcriptional profiles following Mycobacterium avium subsp. paratuberculosis infection in mice

Project description:We focused on how Mycobacterium avium subsp. paratuberculosis influences the subsequent host response to investigate the host immunopathology accompanying the host anti-mycobacterial immune response during Mycobacterium avium subsp. paratuberculosis infection in spleen of mice. We analyzed altered transcription in the spleen of mice at 3, 6, and 12 weeks following Mycobacterium avium subsp. paratuberculosis infection.

2014-10-31 | E-GEOD-62836 | biostudies-arrayexpress

Transcriptional profiling of whole-blood in Mycobacterium avium subsp. paratuberculosis-infected cattle

Project description:To provide novel insights into understanding the host-immune response during the different stages of progression of the disease, we performed gene expression profiling in Mycobacterium avium subsp. paratuberculosis infection in cattle. We analyzed the transcriptional profiles of whole-blood cells of cattle identified and grouped according to the presence of MAP-specific antibodies and the MAP shed by them. The grouping was based on the results obtained by ELISA and PCR analyses as follows: 1) Test1 group: MAP-negative results obtained by ELISA and positive results obtained by PCR; 2) Test2 group: MAP-positive results obtained by ELISA and negative results obtained by PCR; 3) Test3 group: MAP-positive results obtained by ELISA and positive results obtained by PCR; 4) uninfected control: MAP-negative results obtained both by ELISA and PCR analysis.

2014-10-31 | E-GEOD-62835 | biostudies-arrayexpress

miRNA profiling of putative biomarker candidates in Mycobacterium avium subsp. paratuberculosis-infected cattle

Project description:Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease that is a chronic wasting disease in ruminants. Spread of MAP is mainly provoked by a long subclinical stage during which MAP is shed into the environment with feces; accordingly, detection of subclinical animals is very important to its control. Therefore, the current diagnostic methods are not suitable for detection of subclinical animals. In this study, we conducted to develop a diagnostic method for analysis of the expression of genes of prognostic potential biomarker candidates in cattle naturally infected with MAP. We conducted miRNA-Seq to evaluate potential biomarker candidates. Experimental animals were divided into four groups based on fecal MAP PCR, serum ELISA, and clinical sign.

2021-02-05 | GSE166230 | GEO

Transcription profiling by array of Mycobacterium avium subsp. paratuberculosis cattle and sheep strains grown under in vitro iron limiting or iron sufficient conditions

Project description:Transcriptional responses of two strain types of Mycobacterium avium subsp. paratuberculosis (MAP, cattle and sheep Strain) under in vitro iron limiting or iron sufficient growth conditions. Background: In MAP, the transcriptional role and essentiality of MAP2827 (IdeR) as an iron dependent regulator has been well established (Janagama et al. 2009). Therefore, in the absence of an ideR deletion mutant of MAP, to understand the genome-wide iron dependent transcriptional variations between the cattle and sheep MAP IdeR we used a heterologous expression of MAP IdeR in Mycobacterium smegmatis ideR deletion mutant.

2010-10-31 | E-MEXP-2865 | biostudies-arrayexpress

Mycobacterium avium subsp. paratuberculosis antigens induce cellular immune responses in cattle

Project description:Mycobacterium avium subspecies paratuberculosis (MAP) causes chronic progressive granulomatous enteritis leading to diarrhea, weight loss, and eventual death in ruminants. Commercially available vaccines provide only partial protection against MAP infection and can interfere with the use of current diagnostic tests for bovine tuberculosis in cattle. In the current study, we characterized immune responses in calves to vaccines containing either MAP fusion protein particles (MAP antigens Ag85A202-347-SOD1-72 -Ag85B173-330-74F1-148+669-786 as a fusion), recombinant MAP (rMAP) fusion protein or commercially available Silirum(R) vaccine.

2022-12-21 | GSE221544 | GEO

Identification of immunoreactive proteins of Mycobacterium avium subsp. Paratuberculosis

Project description:Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of a chronic enteritis of ruminants (bovine paratuberculosis-Johne disease) that is associated with enormous worldwide economic losses for the zootechnical industries. Diagnosis is based on observation of clinical signs, on the detection of antibodies in milk or serum or on evaluation of bacterial culture from feces. The limit of these methods is that they are not able to detect the disease in the subclinical stage and are applicable only when the disease is already in an advanced status. For this reason the main purpose of this study is to use the MAP proteome to detect novel immunoreactive proteins that may be helpful for paratuberculosis diagnoses. 2D electrophoresis and 2D immunoblotting of MAP proteins were performed using sera of control cattle and paratuberculosis infected cattle in order to highlight the specific immunoreactive proteins. Among the assigned identifiers to immunoreactive spots it was found that most of them correspond to surface-located proteins while three of them have never been described before as antigens. The identification of these proteins improves scientific knowledge that could be useful for paratuberculosis diagnoses. The sequence of the identified protein can be used for the synthesis of immunoreactive peptides that could be screened for their immunoreaction against bovine sera infected with MAP.

2014-11-20 | PXD001159 | Pride

Reanalysis of Data-dependent acquisition based proteomic datasets of Mycobacterium avium subsp. paratuberculosis, Mycobacterium marinum and Mycobacterium vaccae

Project description:Detection of species-specific proteotypic peptides for accurate and easy characterization of infectious non-tuberculous mycobacteria such as Mycobacterium avium subsp. paratuberculosis, Mycobacterium marinum and Mycobacterium vaccae is essential. Therefore, we carried out reanalysis of publicly available M. avium subsp. paratuberculosis, M. marinum and M. vaccae proteomic dataset PXD027444, PXD003766 and PASS00954 by proteome database search and followed by spectral library generation. The raw DDA data were searched against their respective reference proteome databases using Proteome Discoverer and FragPipe. The resulting peptide spectrum matches were converted into a spectral library using BiblioSpec.

2024-03-07 | PXD045965 | Pride

Data-dependent acquisition based proteomic analysis of Mycobacterium avium subsp. paratuberculosis ATCC 19698

Project description:Detection of species-specific proteotypic peptides for accurate and easy characterization of infectious non-tuberculous mycobacteria such as Mycobacterium avium subsp. paratuberculosis is essential. Therefore, we conducted an in-depth global proteomic experiment using M. avium subsp. paratuberculosis ATCC 19698 (Map) strain followed by proteome database search and spectral library generation. The lysate was subjected to in-solution proteomic sample preparation and fractionated using an offline C18 StageTip. Each fraction was acquired in technical triplicates using a 180 min data-dependent acquisition (DDA) method in Orbitrap Fusion Tribrid (Thermo Scientific) mass spectrometer. The resulting raw DDA data were searched against the M. avium subsp. paratuberculosis proteome database using Proteome Discoverer and FragPipe. The resulting peptide spectrum matches were converted into a spectral library using BiblioSpec.

2024-03-07 | PXD040589 | Pride

Exploring transcriptional regulation of Mycobacterium avium subsp. paratuberculosis after human THP-1 cell infection via dual RNA-seq analysis

Project description:Host-pathogen interactions of Mycobacterium avium subsp. paratuberculosis infection at transcriptomic level were investigated in an in vitro macrophage infection model by dual RNA-seq analysis.

2022-09-21 | GSE199476 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data