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SGBS adipocytes overexpressing miR-192* or a non-targeting control miRNA

ABSTRACT: To determine whether expression microRNAs (miRNAs) in visceral adipose tissue (VAT) is altered in human non-alcoholic fatty liver disease (NAFLD), we first performed miRNA profiling of VAT from subjects with (n=15, macroscopic liver fat >30%) or without (<5%, non-NAFLD, n=15) NAFLD. VAT miRNA expression profiles in the two groups were similar except for a single miRNA, miR-192*, which was 1.9-fold increased in NAFLD. We next overexpressed miR-192* in cultured human adipocytes, measured its effect on the cellular triglyceride content, and characterized these adipocytes by transcriptomic profiling followed by pathway analysis, qPCR and quantification of select cellular proteins.

ORGANISM(S): Homo sapiens

PROVIDER: GSE62951 | GEO | 2014/11/05

SECONDARY ACCESSION(S): PRJNA266280

REPOSITORIES: GEO

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