Transcriptomics,Genomics

Dataset Information

55

Investigating heterogeneity within T follicular helper cells (TFH) population.


ABSTRACT: T follicular helper cells (TFH) are heterogenic population of CD4+ T cells, expressing CXCR5+ and PD-1+ on their surface. Their role is linked to supporting formation of germinal centres (GC) and these cells are thought to express high levels of PD-1 marker. Two models of immunisation were used to investigate the role of PD-1 low TFH. In Salmonella enterica infection high frequency of T follicular helper cells expressing low levels of PD-1 surface molecule are observed within first week of infection but GC do not appear until much a later stage (week 7-8). Sheep red blood cell immunisation (SRBC) gives rise to both TFH and GC B cells within first week of response and these TFH express low to high level of PD-1 molecule. Microarray analysis was applied in order to investigate the relationship between and characteristics of different TFH populations in order to elucidate their role in GC support, developmental stage and contributions to memory T cell pool. Overall design: Extensive FACS sorting was undertaken to separate populations of TFH from S. enterica or SRBC immunised mice (day 6 p.i.) form the effector cells. In SRBC model TFH cells were further separated into PD-1 high and PD-1 low TFH, based on the expression of PD-1 surface molecule. As a control population, effector cells were isolated from immunised mice and naive CD4+ T cells from naive mice.

INSTRUMENT(S): [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] [CDF: MoGene11stv1_Mm_ENTREZG_13.0]

SUBMITTER: Alasdair Ivens  

PROVIDER: GSE62961 | GEO | 2015-12-25

SECONDARY ACCESSION(S): PRJNA266300

REPOSITORIES: GEO

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Publications

Heterogeneity of Phenotype and Function Reflects the Multistage Development of T Follicular Helper Cells.

Trüb Marta M   Barr Tom A TA   Morrison Vicky L VL   Brown Sheila S   Caserta Stefano S   Rixon Jordan J   Ivens Alasdair A   Gray David D  

Frontiers in immunology 20170428


T follicular helper cells (Tfh) provide crucial signals for germinal center (GC) formation, but Tfh populations are heterogeneous. While PD1hi Tfh are important in the GC response, the function of the PD1lo Tfh-like subset is unknown. We show that these cells, like the PD1hi GC-Tfh, depend upon B cells; however, their entry to follicles is independent of CXCR5 or cognate interactions with B cells. The differentiation into PD1hi Tfh is dependent on MHC class II interactions with B cells and requi  ...[more]

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