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High-resolution Sox2 DNA-binding sites mapping by ChIP-exo in TSA-treated ES cells

ABSTRACT: Mapping ultra-high resolution of Sox2:DNA interaction after trichostatin A (TSA) treatment would provide us with valuable new mechanistic insights into how epigenome regulates transcription factor DNA interactions in the cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE62972 | GEO | 2014/12/01

SECONDARY ACCESSION(S): PRJNA266322

REPOSITORIES: GEO

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Project description:The goal of this study is to identify the DNA methylation changes caused by exposure of to the DNMT inhibitor 5-aza-2â-deoxycytidine (5Aza) and HDAC inhibitor Trichostatin A (TSA). We performed whole-genome bisulfite sequencing of the drug-treated MCF7 breast cancer cell lines and compare their DNA methylation profile with the untreated MCF7 (see E-MTAB-2014). While MCF7 treated with both drugs experienced global loss of DNA methylation, the 5Aza induced stronger demethylation than TSA.

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High-resolution Brachyury DNA-binding Sites Mapping by ChIP-exo

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